Riviere Y, Ahmed R, Southern P J, Buchmeier M J, Oldstone M B
J Virol. 1985 Sep;55(3):704-9. doi: 10.1128/JVI.55.3.704-709.1985.
The Armstrong CA 1371 (ARM) and WE strains of lymphocytic choriomeningitis virus (LCMV) differ in the ability to produce disease in adult guinea pigs. Infection with the ARM strain is not lethal, even at high virus doses (greater than 10,000 PFU), whereas the WE strain causes 100% mortality even at low doses (less than 10 PFU). To determine the genetic basis of this virulence, intertypic reassortants were made between the ARM and WE strains of LCMV. The two reassortants with the genotypes WE/ARM (L segment of WE and S segment of ARM) and ARM/WE (L segment of ARM and S segment of WE) were tested for their pathogenicity in guinea pigs. The ARM/WE reassortant was avirulent like the ARM/ARM parental strain. Minimal viral replication was observed in organs of guinea pigs inoculated with 10(2) or 10(5) PFU of ARM/ARM or ARM/WE, and all animals survived. In contrast, the WE/ARM reassortant was highly virulent like the WE/WE parental strain and killed all of the infected animals. High levels of viral replication were observed in guinea pigs infected with the latter two strains. In contrast to these in vivo observations, both the parental strains and the ARM/WE or WE/ARM reassortants had similar growth potential in cultured guinea pig fibroblasts. Thus, the L RNA segment of LCMV WE is important for viral replication in vivo and is associated with fatal acute disease after infection of adult guinea pigs.
淋巴细胞性脉络丛脑膜炎病毒（LCMV）的阿姆斯特朗CA 1371（ARM）株和WE株在成年豚鼠中引发疾病的能力有所不同。用ARM株感染即使在高病毒剂量（大于10,000 PFU）时也不会致死，而WE株即使在低剂量（小于10 PFU）时也会导致100%的死亡率。为了确定这种毒力的遗传基础，在LCMV的ARM株和WE株之间构建了异型重配病毒。对两种基因型为WE/ARM（WE的L片段和ARM的S片段）和ARM/WE（ARM的L片段和WE的S片段）的重配病毒进行了豚鼠致病性测试。ARM/WE重配病毒与ARM/ARM亲代毒株一样无毒力。在用10²或10⁵ PFU的ARM/ARM或ARM/WE接种的豚鼠器官中观察到极少的病毒复制，所有动物均存活。相比之下，WE/ARM重配病毒与WE/WE亲代毒株一样具有高毒力，感染的动物全部死亡。在感染后两种毒株的豚鼠中观察到高水平的病毒复制。与这些体内观察结果相反，亲代毒株以及ARM/WE或WE/ARM重配病毒在培养的豚鼠成纤维细胞中具有相似的生长潜力。因此，LCMV WE的L RNA片段对于体内病毒复制很重要，并且与成年豚鼠感染后的致命急性疾病相关。
