一种不同型淋巴细胞性脉络丛脑膜炎重组病毒的形成与特性分析

Formation and characterization of an intertypic lymphocytic choriomeningitis recombinant virus.

作者信息

Kirk W E, Cash P, Peters C J, Bishop D H

出版信息

J Gen Virol. 1980 Nov;51(Pt 1):213-8. doi: 10.1099/0022-1317-51-1-213.

Abstract

An intertypic reassortant arenavirus has been obtained from co-infection of BHK-21 cells by two strains of lymphocytic choriomeningitis virus (LCM virus), WE and Armstrong (ARM), having the large/small (L/S) viral RNA genotype of WE/ARM. The two parental viruses have different virulence characteristics in hamsters and guniea-pigs. The reassortant virus induces LCM-WE-type plaques in Vero cell monolayers, but has the avirulent characteristics of LCM-ARM in hamsters and two strains of guniea-pigs. These results suggest that the LCM plaque phenotype is determined by L RNA gene products, while the pathogenic potential in guinea-pigs may be determined by S RNA gene products.

摘要

一株不同型间重配的沙粒病毒是通过两株淋巴细胞性脉络丛脑膜炎病毒（LCM病毒），即WE株和阿姆斯特朗（ARM）株共同感染BHK-21细胞而获得的，其具有WE/ARM的大/小（L/S）病毒RNA基因型。这两种亲代病毒在仓鼠和豚鼠中具有不同的毒力特征。该重配病毒在Vero细胞单层中诱导出LCM-WE型蚀斑，但在仓鼠和两株豚鼠中具有LCM-ARM的无毒力特征。这些结果表明，LCM蚀斑表型由L RNA基因产物决定，而在豚鼠中的致病潜力可能由S RNA基因产物决定。