Li Xiao, Wang Shuang, Nie Xin, Hu Yuexin, Liu Ouxuan, Wang Yuxuan, Lin Bei
Department of Gynecology and Obstetrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.
Department of Obstetrics and Gynaecology, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin 300277, P.R. China.
Int J Mol Med. 2025 Jun;55(6). doi: 10.3892/ijmm.2025.5529. Epub 2025 Apr 11.
Epithelial ovarian cancer, the most prevalent form of ovarian cancer, is a health concern worldwide. Phosphoserine aminotransferase 1 (PSAT1), as the rate‑limiting enzyme in serine synthesis, is key in the conversion of 3‑phosphoglycerate to serine. The present study explored the role of PSAT1 expression in epithelial ovarian tumors. Gene Expression Profiling Interactive Analysis was used for gene expression and survival analyses. The effects of PSAT1 overexpression and knockdown on invasion, migration, proliferation and cell cycle progression of ovarian cancer cell lines were investigated both and . Western blotting was conducted to assess alterations in PI3K/AKT signalling pathway proteins. Database and tissue sample data confirmed that PSAT1 was significantly upregulated in ovarian cancer. Preliminary functional investigations indicated that PSAT1 was involved in modulation of invasion and migration, demonstrating the capacity of PSAT1 to enhance expression of the PI3K/AKT signalling pathway. These findings suggested that PSAT1 served a critical role in the onset and progression of ovarian cancer, thereby offering a theoretical basis for early detection and therapeutic strategies.
上皮性卵巢癌是卵巢癌最常见的形式，是全球关注的健康问题。磷酸丝氨酸转氨酶1（PSAT1）作为丝氨酸合成中的限速酶，在3-磷酸甘油酸转化为丝氨酸的过程中起关键作用。本研究探讨了PSAT1表达在上皮性卵巢肿瘤中的作用。利用基因表达谱交互分析进行基因表达和生存分析。通过体内和体外实验研究了PSAT1过表达和敲低对卵巢癌细胞系侵袭、迁移、增殖和细胞周期进程的影响。进行蛋白质免疫印迹法以评估PI3K/AKT信号通路蛋白的变化。数据库和组织样本数据证实PSAT1在卵巢癌中显著上调。初步功能研究表明，PSAT1参与侵袭和迁移的调节，表明PSAT1具有增强PI3K/AKT信号通路表达的能力。这些发现提示PSAT1在卵巢癌的发生和发展中起关键作用，从而为早期检测和治疗策略提供理论依据。
