Naseem Sobia, Rizwan Muhammad, Al-Qahtani Wahidah H, Sadiqa Ayesha, Ahmad Awais
Department of Polymer & Process Engineering, University of Engineering and Technology Lahore, Pakistan.
Department of Chemistry, University of Engineering and Technology Lahore, Pakistan.
Food Chem X. 2025 Mar 19;27:102374. doi: 10.1016/j.fochx.2025.102374. eCollection 2025 Apr.
Recycling food waste to extract valuable bioactive compounds (BAC) offers a groundbreaking solution to mitigate environmental challenges. The isolation and purification of BAC from food waste are getting more attention to maintain environmental sustainability. The therapeutic potential of these compounds highlights the need for innovative approaches to extracting and purifying them from by-products. In this context, the chlorogenic (CG) acid is extracted from watermelon rind (WMR) using ultrasound-assisted extraction (USAE) technique and purified using the macroporous adsorption resin (MPAR) method. The extraction and purification were carried out with water, 70 % /v ethanol/water (70 % Et), and 90 % v/v ethanol/water (90 %-Et) solvent to analyze the impact of solvent on extraction efficiency, purity, and recovery. The extracted CG acid was qualitatively analyzed via UV spectrometry and HPLC and underwent FESEM analysis while purified CG acid was characterized via FTIR and thermal analysis. The implementation of the MPAR method notably increased the yield to 4.6 mg/g of raw material using solvents comprising 90 % ethanol than water and 70 % ethanol. The recovery of CG acid significantly escalated to 88 %, enhancing the purity percentage from an initial range of 70-94 %.
将食物垃圾回收以提取有价值的生物活性化合物（BAC）为缓解环境挑战提供了一种开创性的解决方案。从食物垃圾中分离和纯化BAC越来越受到关注，以维持环境可持续性。这些化合物的治疗潜力凸显了从副产品中提取和纯化它们的创新方法的必要性。在此背景下，使用超声辅助提取（USAE）技术从西瓜皮（WMR）中提取绿原酸（CG），并采用大孔吸附树脂（MPAR）法进行纯化。分别用水、70%（v/v）乙醇/水（70% Et）和90%（v/v）乙醇/水（90%-Et）溶剂进行提取和纯化，以分析溶剂对提取效率、纯度和回收率的影响。通过紫外光谱法和高效液相色谱法对提取的CG酸进行定性分析，并进行场发射扫描电子显微镜（FESEM）分析，而纯化的CG酸则通过傅里叶变换红外光谱（FTIR）和热分析进行表征。与水和70%乙醇相比，采用含90%乙醇的溶剂时，MPAR法的实施显著提高了产率，达到4.6 mg/g原料。CG酸的回收率显著提高到88%，纯度从初始的70-94%提高。
