Luque-Alcaraz Ana Guadalupe, Maldonado-Arriola Jesús Antonio, Hernández-Abril Pedro Amado, Álvarez-Ramos Mario Enrique, Hernández-Téllez Cynthia Nazareth
Department of Biomedical Engineering, Universidad Estatal de Sonora, Hermosillo 83100, Mexico.
Department of Industrial Engineering, Universidad de Sonora, Hermosillo 83000, Mexico.
Nanomaterials (Basel). 2025 Apr 2;15(7):539. doi: 10.3390/nano15070539.
This study investigates the synthesis and characterization of zein nanoparticles (ZNp) loaded with grape pomace extract (GPE) from L. for applications in controlled release and antioxidant delivery. Grape pomace, a byproduct of the winemaking industry, is rich in bioactive compounds, including phenols and flavonoids, which possess antioxidant properties. To overcome the limitations of these compounds, such as photosensitivity and thermal degradation, they were incorporated into zein nanoparticles using the antisolvent technique. The physicochemical properties of the ZNp-GPE system were thoroughly characterized, including size, morphology, ζ-potential, and total phenol content. Results showed high encapsulation efficiency (89-97%) and favorable loading capacities. Characterization techniques, such as scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS), confirmed that GPE was successfully incorporated into the nanoparticles, thereby enhancing their antioxidant properties. The encapsulation process did not significantly alter the spherical morphology of the nanoparticles, and loading GPE resulted in a decrease in particle size. Total phenol content analysis showed that the ZNp-GPE nanoparticles effectively retained these compounds, confirming their potential as efficient delivery systems for antioxidants. This approach not only provides a method for protecting and enhancing the bioavailability of natural antioxidants but also contributes to the valorization of agricultural waste, promoting sustainability in bio-based industries.
本研究考察了负载葡萄皮渣提取物（GPE）的玉米醇溶蛋白纳米颗粒（ZNp）的合成与表征，该提取物来源于酿酒葡萄，旨在用于控释和抗氧化剂递送。葡萄皮渣是酿酒工业的副产品，富含生物活性化合物，包括具有抗氧化特性的酚类和黄酮类化合物。为克服这些化合物的局限性，如光敏性和热降解，采用反溶剂技术将它们掺入玉米醇溶蛋白纳米颗粒中。对ZNp-GPE体系的物理化学性质进行了全面表征，包括尺寸、形态、ζ电位和总酚含量。结果显示包封率高（89-97%）且负载量良好。扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和动态光散射（DLS）等表征技术证实，GPE已成功掺入纳米颗粒中，从而增强了它们的抗氧化性能。包封过程并未显著改变纳米颗粒的球形形态，负载GPE导致粒径减小。总酚含量分析表明，ZNp-GPE纳米颗粒有效地保留了这些化合物，证实了它们作为抗氧化剂有效递送系统的潜力。这种方法不仅提供了一种保护和提高天然抗氧化剂生物利用度的方法，而且有助于农业废弃物的增值利用，促进生物基产业的可持续发展。
