Sengottaiyan Jayapriya, Parvathi Kalyanasundaram, Ebenezer Anbaison Franklin, Sampathkumar Natarajan, Rajesh Jegathalaprathaban, Rajagopal Gurusamy
Department of Chemistry, Chikkanna Government Arts College, Tirupur, 641602, Tamil Nadu, India.
Department of Chemistry, Sri Shakthi Institute of Engineering and Technology, Coimbatore, 641 062, Tamil Nadu, India.
J Fluoresc. 2025 Apr 11. doi: 10.1007/s10895-025-04299-7.
A diaminomaleonitrile-based fluorescent probe (CZ-DAMN) for the selective detection of Cuwas synthesized and characterized by FTIR, H-NMR,C-NMR, and mass spectral analysis. The absorbance and fluorescent spectra and competition experiments investigated the sensing behavior of CZ-DAMN towards different metal ions. The results showed that the probe exhibited an enhanced emission at 436 nm (λ = 320) with cyan fluorescence in the presence of Cuions over other metal ions in CHCN:HO (8:2, v/v). A 1:1 stoichiometric binding of the probeCZ-DAMN to Cu was observed according to Job's plot analysis. The binding constant was estimated at 7.38 × 10 M with a detection limit of 14.81 nM. Turn-on was attributed to the inhibition of photoinduced electron transfer (PET) and -C = N- isomerization following Cubinding, whereas redshifted absorbance of the free probewas attributed to intramolecular charge transfer (ICT). Several methods were used to study the binding mechanism, including EPR, FTIR, ESI-mass, and DFT/TD-DFT. The probeCZ-DAMN demonstrated the detection of even trace amounts of Cuions in real-time pharmaceutical samples. It was also used for on-site Cu detection using probe-coated dipsticks.
合成了一种基于二氨基马来腈的用于选择性检测铜离子的荧光探针（CZ-DAMN），并通过傅里叶变换红外光谱（FTIR）、氢核磁共振（H-NMR）、碳核磁共振（C-NMR）和质谱分析对其进行了表征。通过吸收光谱、荧光光谱和竞争实验研究了CZ-DAMN对不同金属离子的传感行为。结果表明，在CHCN:HO（8:2，v/v）中，该探针在铜离子存在下于436 nm（λ = 320）处表现出增强的发射，发出青色荧光，优于其他金属离子。根据Job曲线分析，观察到探针CZ-DAMN与铜以1:1的化学计量比结合。结合常数估计为7.38×10 M，检测限为14.81 nM。荧光开启归因于铜离子结合后对光致电子转移（PET）的抑制和-C=N-异构化，而游离探针吸收光谱的红移归因于分子内电荷转移（ICT）。使用了几种方法来研究结合机制，包括电子顺磁共振（EPR）、傅里叶变换红外光谱（FTIR）、电喷雾电离质谱（ESI-质谱）和密度泛函理论/含时密度泛函理论（DFT/TD-DFT）。探针CZ-DAMN能够实时检测实际药物样品中痕量的铜离子。它还被用于使用涂有探针的试纸进行现场铜检测。
