一种基于 'C-CN' 的新型比色和比率荧光探针，用于检测联氨及其在活细胞和小鼠中的成像。
A novel colorimetric and ratiometric fluorescence probe based on 'C-CN' for detection of hydrazine and its imaging in living cells and mouse.
机构信息
College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China.
College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China; Changzhou Vocational Institute of Engineering, Changzhou 213100, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Mar 5;268:120639. doi: 10.1016/j.saa.2021.120639. Epub 2021 Nov 18.
Hydrazine plays an important role in chemistry, pharmaceuticals, agriculture and aerospace. However, it is not to be underestimated and has been identified as harmful to the human body. Therefore, it is significant and urgent to develop the detection of hydrazine in vivo and in vitro. Here, the probe TAN was synthesized by using benzothiazole derivatives as the fluorophore and 2,3-diaminomaleonitrile as the identified group to detect hydrazine. The presence of hydrazine resulted in a colorimetric and ratiometric fluorescence response of the probe based on the formation of hydrazone. The detection limit of TAN was 0.31 µM for hydrazine. In addition, the probe TAN was successfully used to visualize hydrazine in living HepG-2 cells and mouse with low cytotoxicity and excellent biocompatibility.
水合肼在化学、制药、农业和航空航天等领域发挥着重要作用。然而，它的危害不容小觑，已被确定对人体有害。因此，开发体内和体外水合肼的检测方法具有重要意义且迫在眉睫。本文以苯并噻唑衍生物为荧光团，2,3-二氨基马来腈为识别基团，合成了探针 TAN，用于检测水合肼。水合肼的存在导致探针基于腙的形成产生比色和比率荧光响应。TAN 对水合肼的检测限为 0.31µM。此外，该探针 TAN 还成功地用于可视化活 HepG-2 细胞和小鼠中的水合肼，具有低细胞毒性和优异的生物相容性。
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