Suzuki M, Azegami M
Biochim Biophys Acta. 1977 Feb 16;474(4):646-61. doi: 10.1016/0005-2787(77)90084-3.
Hydroxylamine-resistant infectious materials (HARIM) synthesized in natural non-host and progeny phage low productive bacterial spheroplasts upon transfection with bacteriophage phiX174 DNA were found to be unusually heterogeneous in their forms. Using Pseudomonas aeruginosa as a source of HARIM, it was shown that they have the following unusual features. (1) Almost all of the HARIM are denser than normal single-stranded (SS)- and double-stranded replicative form (RF)-DNAs of phiX174 found usually in the phage-infected host cells. (2) A great part of these heavy HARIM (approximately 84%) contain a variable length of single-stranded RNA associated with their infectious elements. (3) For most of the HARIM (approximately 80% of total molecules as the infectious elements of the heavy HARIM), the infectious elements are phiX-RFI-DNA. The wide-spread system for phiX-HARIM synthesis was shown to be present in many gram-negative bacterial cells.
在用噬菌体phiX174 DNA转染天然非宿主和子代噬菌体低产细菌原生质体时合成的耐羟胺感染性物质（HARIM），其形态异常多样。以铜绿假单胞菌作为HARIM的来源，研究表明它们具有以下异常特征。（1）几乎所有的HARIM都比通常在噬菌体感染宿主细胞中发现的phiX174正常单链（SS）和双链复制型（RF）DNA密度更大。（2）这些重质HARIM中的很大一部分（约84%）含有与它们的感染元件相关的可变长度单链RNA。（3）对于大多数HARIM（约占重质HARIM感染元件总分子数的80%），感染元件是phiX - RFI - DNA。结果表明，phiX - HARIM合成的广泛系统存在于许多革兰氏阴性细菌细胞中。
