Marians K J, Ikeda J E, Schlagman S, Hurwitz J
Proc Natl Acad Sci U S A. 1977 May;74(5):1965-8. doi: 10.1073/pnas.74.5.1965.
Preparations containing DNA gyrase activity Gellert, M., Mizuchi, K., O'Dea, M.H. & Nash, H.A. (1976) Proc. Natl. Acad. Sci. USA 73, 3872-3876] have been extensively purified from Escherichia coli. Such fractions, in the presence of ATP and Mg2+, catalyze supertwisting of relaxed circular double-stranded DNA replicative forms of a number of DNAs that results in the formation of superhelical replicative forms. Relaxed phiX174 replicative form (phiX RFIV) is not attacked by the A protein endonuclease coded for by the phiX DNA genome. After exposure to preparations of DNA gyrase, the relaxed phiX174 replicative form is converted to phiX RFI which can then be attacked by the phiX gene A protein and participate in replication of duplex phiX DNA.
含有DNA促旋酶活性的制剂[盖勒特，M.，水内，K.，奥戴亚，M.H.和纳什，H.A.（1976年）《美国国家科学院院刊》73，3872 - 3876]已从大肠杆菌中得到广泛纯化。在ATP和Mg2 +存在的情况下，此类组分催化多种DNA的松弛环状双链DNA复制形式的超螺旋化，导致超螺旋复制形式的形成。松弛的φX174复制形式（φX RFIV）不会受到由φX DNA基因组编码的A蛋白核酸内切酶的攻击。在接触DNA促旋酶制剂后，松弛的φX174复制形式会转化为φX RFI，然后它可以被φX基因A蛋白攻击并参与双链φX DNA的复制。
