体外DNA合成依赖于φX174复制型I DNA。
DNA synthesis in vitro dependent upon phiX174 replicative form I DNA.
作者信息
Sumida-Yasumoto C, Yudelevich A, Hurwitz J
出版信息
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1887-91. doi: 10.1073/pnas.73.6.1887.
Abstract
Extracts of Escherichia coli strains infected with bacteriophage phiX174 catalyze DNA synthesis dependent on double-stranded, circular phiX174 replicative form I (phiX RFI) by a semiconservative process. The reaction required Mg++, ATP, all four dNTP, and exogenous phiX RFI DNA as template and yielded phiX RFI and phiX RFII. The reaction was inhibited by nalidixic acid and novobiocin but not by rifampicin. DNA synthesis required the phiX174 gene A product and E. coli gene products dnaB, dnaC(D), dnaG, and rep.
摘要
被噬菌体phiX174感染的大肠杆菌菌株提取物通过半保留过程催化依赖于双链环状phiX174复制型I（phiX RFI）的DNA合成。该反应需要Mg++、ATP、所有四种dNTP以及外源phiX RFI DNA作为模板，并产生phiX RFI和phiX RFII。该反应受到萘啶酸和新生霉素的抑制，但不受利福平的抑制。DNA合成需要phiX174基因A产物以及大肠杆菌基因产物dnaB、dnaC(D)、dnaG和rep。
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