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1
DNA synthesis in vitro dependent upon phiX174 replicative form I DNA.体外DNA合成依赖于φX174复制型I DNA。
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1887-91. doi: 10.1073/pnas.73.6.1887.
2
Synthesis of phiX174 viral DNA in vitro depends on phiX replicative form DNA.在体外合成φX174病毒DNA依赖于φX复制型DNA。
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4195-9. doi: 10.1073/pnas.74.10.4195.
3
Role of DNA gyrase in phiX replicative-form replication in vitro.DNA 回旋酶在体外φX复制型复制中的作用。
Proc Natl Acad Sci U S A. 1977 May;74(5):1965-8. doi: 10.1073/pnas.74.5.1965.
4
Isolation and characterization of the protein coded by gene A of bacteriophage phiX174 DNA.噬菌体φX174 DNA的基因A所编码蛋白质的分离与特性分析
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2669-73. doi: 10.1073/pnas.73.8.2669.
5
Synthesis of alpha3 phage DNA in replication mutants of Escherichia coli.大肠杆菌复制突变体中α3噬菌体DNA的合成
Biochim Biophys Acta. 1978 Jan 26;517(1):55-64. doi: 10.1016/0005-2787(78)90033-3.
6
Rifampin inhibition of bacteriophage phiX174 parental replicative-form DNA synthesis in an Escherichia coli dnaC mutant.利福平对大肠杆菌dnaC突变体中噬菌体phiX174亲本复制型DNA合成的抑制作用。
J Virol. 1975 Sep;16(3):575-80. doi: 10.1128/JVI.16.3.575-580.1975.
7
Mechanism of replication of phi x174 single-stranded DNA. IX. Requirement for the Escherichia coli dnaG protein.φX174单链DNA的复制机制。IX. 对大肠杆菌dnaG蛋白的需求
J Virol. 1974 Nov;14(5):1070-5. doi: 10.1128/JVI.14.5.1070-1075.1974.
8
Association of phiX174 DNA-dependent ATPase activity with an Escherichia coli protein, replication factor Y, required for in vitro synthesis of phiX174 DNA.φX174 DNA 依赖性 ATP 酶活性与一种大肠杆菌蛋白复制因子 Y 的关联，该蛋白是体外合成 φX174 DNA 所必需的。
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3342-6. doi: 10.1073/pnas.72.9.3342.
9
Bacteriophage phiX174 single-stranded viral DNA synthesis in temperature-sensitive dnaB and dna C mutants of Escherichia coli.噬菌体φX174单链病毒DNA在大肠杆菌温度敏感型dnaB和dnaC突变体中的合成
J Virol. 1976 May;18(2):426-35. doi: 10.1128/JVI.18.2.426-435.1976.
10
Conversion of bacteriophage G4 single-stranded viral DNA to double-stranded replicative form in dna mutants of Escherichia coli.噬菌体G4单链病毒DNA在大肠杆菌的dna突变体中转化为双链复制形式。
Biochim Biophys Acta. 1977 May 17;476(2):149-55. doi: 10.1016/0005-2787(77)90091-0.

引用本文的文献

1
Cellular characterization of the primosome and rep helicase in processing and restoration of replication following arrest by UV-induced DNA damage in Escherichia coli.大肠杆菌中紫外线诱导的DNA损伤导致复制停滞后，引发体和rep解旋酶在复制加工和恢复过程中的细胞特征分析
J Bacteriol. 2012 Aug;194(15):3977-86. doi: 10.1128/JB.00290-12. Epub 2012 May 25.
2
Deoxyribonucleic acid replication in permeable and fully viable Escherichia coli cells.在可渗透且完全存活的大肠杆菌细胞中的脱氧核糖核酸复制。
J Bacteriol. 1981 Mar;145(3):1413-6. doi: 10.1128/jb.145.3.1413-1416.1981.
3
Morphogenesis of phi X174: in vitro synthesis of infectious phage from purified viral components.噬菌体φX174的形态发生：从纯化的病毒成分体外合成感染性噬菌体。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7285-9. doi: 10.1073/pnas.78.12.7285.
4
Replication of duplex DNA of phage phi X174 reconstituted with purified enzymes.用纯化酶重建的噬菌体φX174双链DNA的复制
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3322-6. doi: 10.1073/pnas.77.6.3322.
5
In vitro DNA replication of recombinant plasmid DNAs containing the origin of progeny replicative form DNA synthesis of phage phi X174.含有噬菌体φX174子代复制型DNA合成起始位点的重组质粒DNA的体外DNA复制。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5182-6. doi: 10.1073/pnas.77.9.5182.
6
Inhibition of deoxyribonucleic acid gyrase: effects on nucleic acid synthesis and cell division in Escherichia coli K-12.脱氧核糖核酸回旋酶的抑制作用：对大肠杆菌K-12核酸合成及细胞分裂的影响
J Bacteriol. 1980 Apr;142(1):153-61. doi: 10.1128/jb.142.1.153-161.1980.
7
Cloning and characterization of livH, the structural gene encoding a component of the leucine transport system in Escherichia coli.大肠杆菌中亮氨酸转运系统一个组分的结构基因livH的克隆与特性分析
J Bacteriol. 1986 May;166(2):565-73. doi: 10.1128/jb.166.2.565-573.1986.
8
Identification of livG, a membrane-associated component of the branched-chain amino acid transport in Escherichia coli.鉴定livG，大肠杆菌中支链氨基酸转运的膜相关组分。
J Bacteriol. 1985 Sep;163(3):1196-202. doi: 10.1128/jb.163.3.1196-1202.1985.
9
Isolation and characterization of the protein coded by gene A of bacteriophage phiX174 DNA.噬菌体φX174 DNA的基因A所编码蛋白质的分离与特性分析
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2669-73. doi: 10.1073/pnas.73.8.2669.
10
Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.新生霉素和香豆霉素抑制由DNA促旋酶催化的DNA超螺旋化。
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4474-8. doi: 10.1073/pnas.73.12.4474.

本文引用的文献

1
THE ABORTIVE REPLICATION OF PhiX174 DNA IN A RECOMBINATION-DEFICIENT MUTANT OF Escherichia coli.PhiX174噬菌体DNA在大肠杆菌重组缺陷型突变体中的流产复制
Proc Natl Acad Sci U S A. 1967 Mar;57(3):813-20. doi: 10.1073/pnas.57.3.813.
2
MECHANISM OF ACTION OF NALIDIXIC ACID ON ESCHERICHIA COLI.II. INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS.萘啶酸对大肠杆菌的作用机制。二、脱氧核糖核酸合成的抑制
J Bacteriol. 1965 Apr;89(4):1068-74. doi: 10.1128/jb.89.4.1068-1074.1965.
3
Fate of parental phi X174-DNA upon infection of starved thymine-requiring host cells.饥饿的胸腺嘧啶需求型宿主细胞被感染后亲本φX174-DNA的命运
Virology. 1971 Apr;44(1):168-87. doi: 10.1016/0042-6822(71)90163-2.
4
Identification of individual sex-factor DNA strands and their replication during conjugation in thermosensitive DNA mutants of Escherichia coli.在大肠杆菌温度敏感型DNA突变体中，鉴定个体性因子DNA链及其在接合过程中的复制情况。
J Mol Biol. 1971 Sep 28;60(3):413-24. doi: 10.1016/0022-2836(71)90178-1.
5
Preparation and purification of phi X-RF component I.φX-RF组分I的制备与纯化
Biochim Biophys Acta. 1968 Jan 29;155(1):295-8. doi: 10.1016/0005-2787(68)90360-2.
6
The process of infection with bacteriophage phi-X174. XV. Bacteriophage DNA synthesis in abortive infections with a set of conditional lethal mutants.噬菌体φ-X174的感染过程。十五。一组条件致死突变体在流产感染中的噬菌体DNA合成。
J Mol Biol. 1967 Nov 28;30(1):69-80. doi: 10.1016/0022-2836(67)90244-6.
7
Multienzyme systems of DNA replication.DNA复制的多酶系统
Science. 1974 Dec 13;186(4168):987-93. doi: 10.1126/science.186.4168.987.
8
Growth of phages lambda, phiX174, and Ml3 requires the dnaZ (previously dnaH) gene product of Escherichia coli.噬菌体λ、φX174和M13的生长需要大肠杆菌的dnaZ（以前称为dnaH）基因产物。
Biochem Biophys Res Commun. 1974 Dec 11;61(3):1036-42. doi: 10.1016/0006-291x(74)90259-9.
9
Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins.用纯化的大肠杆菌蛋白将φX174病毒DNA转化为双链形式。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4120-4. doi: 10.1073/pnas.71.10.4120.
10
Conversion of the M13 viral single strand to the double-stranded replicative forms by purified proteins.通过纯化蛋白将M13病毒单链转化为双链复制形式。
J Biol Chem. 1974 Jul 10;249(13):3999-4005.

体外DNA合成依赖于φX174复制型I DNA。

DNA synthesis in vitro dependent upon phiX174 replicative form I DNA.

作者信息

Sumida-Yasumoto C, Yudelevich A, Hurwitz J

出版信息

Proc Natl Acad Sci U S A. 1976 Jun;73(6):1887-91. doi: 10.1073/pnas.73.6.1887.

DOI:10.1073/pnas.73.6.1887
PMID:778850
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC430412/
Abstract

Extracts of Escherichia coli strains infected with bacteriophage phiX174 catalyze DNA synthesis dependent on double-stranded, circular phiX174 replicative form I (phiX RFI) by a semiconservative process. The reaction required Mg++, ATP, all four dNTP, and exogenous phiX RFI DNA as template and yielded phiX RFI and phiX RFII. The reaction was inhibited by nalidixic acid and novobiocin but not by rifampicin. DNA synthesis required the phiX174 gene A product and E. coli gene products dnaB, dnaC(D), dnaG, and rep.

摘要

被噬菌体phiX174感染的大肠杆菌菌株提取物通过半保留过程催化依赖于双链环状phiX174复制型I（phiX RFI）的DNA合成。该反应需要Mg++、ATP、所有四种dNTP以及外源phiX RFI DNA作为模板，并产生phiX RFI和phiX RFII。该反应受到萘啶酸和新生霉素的抑制，但不受利福平的抑制。DNA合成需要phiX174基因A产物以及大肠杆菌基因产物dnaB、dnaC(D)、dnaG和rep。