Vittek J, L'Hommedieu D G, Gordon G G, Rappaport S C, Southren A L
Life Sci. 1985 Aug 26;37(8):711-6. doi: 10.1016/0024-3205(85)90540-5.
Simple and sensitive direct RIA for determination of salivary testosterone was developed by using RSL NOSOLVEX TM (125 1) kit produced by Radioassay System Laboratories (Carson, California). In addition, a relationship between salivary and serum free and total testosterone concentrations was studied in randomly selected 45 healthy subjects, 5 females on oral contraceptive pills and 28 hypertensive patients on various treatment regimens. The lowest weight of testosterone detectable by our modified method was equivalent to 1 pg/ml of saliva, taking into account analytical variability. Intra- and interassay coefficients of variation were 5.09 +/- 2.7% and 8.2 +/- 5.9% respectively. Statistically significant correlations were found between salivary and serum free testosterone (r = 0.97) and salivary and serum total testosterone concentrations (r = 0.70-0.87). The exception to this was a group of hypertensive females in which no correlation (r = 0.14) between salivary and total serum testosterone was found. It is also of interest that, while salivary testosterone was significantly increased in subjects taking oral contraceptives and most of the hypertensive patients the total serum testosterone concentration was in normal range. Our findings suggest that determination of salivary testosterone is a reliable method to detect changes in the concentration of available biologically active hormone in the circulation.
利用放射免疫分析系统实验室（加利福尼亚州卡森市）生产的RSL NOSOLVEX TM（125 I）试剂盒，开发了一种简单且灵敏的直接放射免疫分析法，用于测定唾液睾酮。此外，还对45名随机选取的健康受试者、5名服用口服避孕药的女性以及28名接受各种治疗方案的高血压患者的唾液和血清中游离睾酮及总睾酮浓度之间的关系进行了研究。考虑到分析变异性，我们改进后的方法可检测到的最低睾酮重量相当于唾液中1 pg/ml。批内和批间变异系数分别为5.09±2.7%和8.2±5.9%。在唾液和血清游离睾酮（r = 0.97）以及唾液和血清总睾酮浓度（r = 0.70 - 0.87）之间发现了具有统计学意义的相关性。唯一的例外是一组高血压女性，在她们的唾液和血清总睾酮之间未发现相关性（r = 0.14）。同样有趣的是，虽然服用口服避孕药的受试者和大多数高血压患者的唾液睾酮显著升高，但血清总睾酮浓度却在正常范围内。我们的研究结果表明，测定唾液睾酮是检测循环中可用生物活性激素浓度变化的可靠方法。
