Chaves Andreia A M, Martins Cátia F, Ribeiro David M, Maia Margarida R G, Fonseca António J M, Cabrita Ana R J, Alves Susana P, Mourato Miguel P, Pinho Mário, Bessa Rui J B, Almeida André M de, Freire João P B
LEAF-Linking Landscape, Environment, Agriculture and Food Research Center, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
Associate Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
Animals (Basel). 2025 Mar 27;15(7):967. doi: 10.3390/ani15070967.
Twenty-four male piglets were randomly assigned to four dietary treatments, with increasing sp. incorporation levels (0%, 5%, 10%, and 15%). Following a 4-day adaptation period to metabolic cages, the animals were used in a 2-week digestibility trial and slaughtered for digestive tract measurements and sampling. The apparent total tract digestibility (ATTD), N balance, small intestine digesta viscosity and histomorphology, and hindgut digesta volatile fatty acid (VFA) profile were determined. Polynomial contrasts were employed to examine linear and quadratic effects of sp. dietary incorporation. The ATTD of most macronutrients and N retention efficiencies decreased linearly ( < 0.05) with sp. dietary inclusion. The ileum villi height increased linearly ( < 0.001) and the hindgut VFA concentration increased linearly ( < 0.05) with dietary sp. inclusion. The ATTD values estimated for sp. biomass using the regression method were 68.3% ± 3.86 for dry matter (DM), 66.1% ± 5.11 for N, and 61.3% ± 4.28 for gross energy. The values calculated for digestible and metabolizable energy (MJ/kg DM) and digestible crude protein (% DM) for sp. were 9.0, 8.8, and 18.3, respectively. sp. biomass had lower ATTD values when compared to protein sources commonly used in swine nutrition.
24只雄性仔猪被随机分配到四种日粮处理组，随着特定物质掺入水平的增加（0%、5%、10%和15%）。在适应代谢笼4天后，将这些动物用于为期2周的消化率试验，然后屠宰以进行消化道测量和取样。测定了表观全肠道消化率（ATTD）、氮平衡、小肠食糜粘度和组织形态学以及后肠食糜挥发性脂肪酸（VFA）谱。采用多项式对比来检验特定物质日粮掺入的线性和二次效应。随着特定物质日粮添加量的增加，大多数常量营养素的ATTD和氮保留效率呈线性下降（P<0.05）。随着日粮中特定物质的添加，回肠绒毛高度呈线性增加（P<0.001），后肠挥发性脂肪酸浓度呈线性增加（P<0.05）。使用回归方法估计的特定物质生物量的ATTD值，干物质（DM）为68.3%±3.86，氮为66.1%±5.11，总能为61.3%±4.28。特定物质的可消化能、代谢能（MJ/kg DM）和可消化粗蛋白（%DM）计算值分别为9.0、8.8和18.3。与猪营养中常用的蛋白质来源相比，特定物质生物量的ATTD值较低。
