Qian Ying, Yu Haihang, Lu Siyu, Bai Yun, Meng Yuan, Chen Lifei, Wu Lin, Zhou Yunwei
College of Horticulture, Jilin Agricultural University, Changchun 130118, China.
Plants (Basel). 2025 Apr 1;14(7):1082. doi: 10.3390/plants14071082.
Drought stress is a significant environmental factor that can impede plant growth and ornamental quality. , a drought-tolerant garden plant, has attracted attention for its ornamental value and application prospects. To investigate the molecular mechanism of drought stress resistance of . , this study employed 20% polyethylene glycol (PEG) 6000 to simulate drought stress. Leaves and roots of . were subjected to 24 h treatment and followed by transcriptome sequencing. Analysis revealed 8796 and 3401 differentially expressed genes (DEGs) in leaves and roots. The major biological processes and key molecular pathways activated under drought stress in . were revealed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. The focus of this analysis was on the gene expression changes within plant hormone signal transduction pathway. Additionally, drought-associated transcription factor families such as AP2/ERF, WRKY, MYB, bHLH, NAC, and bZIP were identified among DEGs. Furthermore, potential regulatory relationships of the above transcription factors (TFs) with functional genes in the abscisic acid (ABA) and jasmonic acid (JA) signalling pathways were analysed using correlation network prediction. This research establishes the groundwork for subsequent exploration of drought-responsive gene expression and regulatory patterns in . and provides an importance for the systematic study of its drought-resistant molecular mechanism.
干旱胁迫是一个重要的环境因素，会阻碍植物生长和观赏品质。[植物名称]作为一种耐旱园林植物，因其观赏价值和应用前景而备受关注。为了研究[植物名称]抗旱的分子机制，本研究采用20%聚乙二醇（PEG）6000模拟干旱胁迫。对[植物名称]的叶片和根系进行24小时处理，然后进行转录组测序。分析显示，叶片和根系中分别有8796个和3401个差异表达基因（DEG）。通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析，揭示了[植物名称]在干旱胁迫下激活的主要生物学过程和关键分子途径。该分析的重点是植物激素信号转导途径内的基因表达变化。此外，在差异表达基因中鉴定出了与干旱相关的转录因子家族，如AP2/ERF、WRKY、MYB、bHLH、NAC和bZIP。此外，利用相关网络预测分析了上述转录因子（TF）与脱落酸（ABA）和茉莉酸（JA）信号通路中功能基因的潜在调控关系。本研究为后续探索[植物名称]中干旱响应基因的表达和调控模式奠定了基础，并为其抗旱分子机制的系统研究提供了重要依据。
