利用基因编码电压指示剂（GEVIs）在群体和细胞水平上绘制突触活动图谱。
Mapping Synaptic Activity at the Population and Cellular Levels with Genetically Encoded Voltage Indicators (GEVIs).
作者信息
Jung Younginha, Nakajima Ryuichi, Ahn Sung Min, Frankiv Nazarii, Lee Haeun, Im Maesoon, Song Yoon-Kyu, Baker Bradley J
机构信息
Bioimaging Data Curation Center, Ewha Womans University, Seoul, Republic of Korea.
Brain Science Division, Korea Institute of Science and Technology, Seoul, Republic of Korea.
出版信息
Methods Mol Biol. 2025;2910:239-251. doi: 10.1007/978-1-0716-4446-1_14.
In this chapter, we provide examples of using genetically encoded voltage indicators (GEVIs) to monitor neuronal intercellular communications at the population level (hippocampus of CA1 region) and individual cell level (retinal ganglion cells). Providing an optical readout for voltage transients, GEVIs enable the reporting of synaptic activity, both activation and inhibition, from chemical and electrical synapses. With the added flexibility of restricting expression of the GEVI to distinct cell types, GEVIs are becoming a powerful tool for interrogating neuronal activity.
在本章中，我们提供了一些使用基因编码电压指示剂（GEVIs）在群体水平（CA1区海马体）和单个细胞水平（视网膜神经节细胞）监测神经元细胞间通讯的示例。GEVIs为电压瞬变提供光学读数，能够报告化学突触和电突触的突触活动，包括激活和抑制。由于具有将GEVI的表达限制在不同细胞类型的额外灵活性，GEVIs正成为研究神经元活动的强大工具。
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