纳米硒减轻了牦牛睾丸间质细胞的免疫反应、细胞凋亡和氧化应激。

Nano-selenium alleviated immunoresponse, apoptosis and oxidative stress in Leydig cells of yak.

作者信息

Li Hao, Tang Yujun, Wang Hui, Liu Xinyue, Zeng Yutian, Zhang Run, Yang Cuiting, Khan Arab, Wu Bing, Wang Xianxiang, Zhang Ming

机构信息

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

College of Science, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.

出版信息

Colloids Surf B Biointerfaces. 2025 Aug;252:114684. doi: 10.1016/j.colsurfb.2025.114684. Epub 2025 Apr 9.

DOI:10.1016/j.colsurfb.2025.114684

PMID:40222115

Abstract

Nano-selenium(SENP) plays a crucial role in maintaining cellular redox homeostasis and serves as an antioxidant in cell culture medium. This study investigated the cytoprotective effects of SENP against lipopolysaccharide (LPS)-induced toxicity in yak Leydig cells. In this research, in vitro cultured Leydig cells were exposed to LPS to simulate Gram-negative bacterial infection. Following LPS induction, the cell apoptosis rate reached 28 %, with significant increases in inflammation and oxidative stress markers including IL-6, IL-8, MDA, and ROS. Concurrently, testosterone concentration decreased by nearly 60 %. Subsequently, SENP was introduced into the culture medium. We then evaluated apoptosis, oxidative stress, immune response, and testosterone concentration in Leydig cells. The results demonstrated that SENP effectively protected Leydig cells from LPS-induced damage.

摘要

纳米硒（SENP）在维持细胞氧化还原稳态中起关键作用，并在细胞培养基中作为抗氧化剂。本研究调查了纳米硒对脂多糖（LPS）诱导的牦牛睾丸间质细胞毒性的细胞保护作用。在本研究中，体外培养的睾丸间质细胞暴露于脂多糖以模拟革兰氏阴性细菌感染。脂多糖诱导后，细胞凋亡率达到28%，炎症和氧化应激标志物包括白细胞介素-6、白细胞介素-8、丙二醛和活性氧显著增加。同时，睾酮浓度下降了近60%。随后，将纳米硒引入培养基中。然后我们评估了睾丸间质细胞中的细胞凋亡、氧化应激、免疫反应和睾酮浓度。结果表明，纳米硒有效地保护睾丸间质细胞免受脂多糖诱导的损伤。