Song Lingmin, Wang Xue, Wang Gang, Zheng Liwei, Zhou Zhansong
Department of Urologic Surgery, Ningbo Yinzhou No. 2 Hospital, Ningbo, China.
Urological Research Institute of People's Liberation Army, Southwest Hospital, Army Medical University, Chongqing, China.
Cancer Rep (Hoboken). 2025 Apr;8(4):e70178. doi: 10.1002/cnr2.70178.
The primary aim of this research is to identify and describe the distinct patterns of microRNAs (miRNAs) that are unusually expressed in benign prostatic hyperplasia (BPH) tissues compared to normal prostatic tissues.
The investigation began with the collection of three samples each from normal prostatic and BPH tissues. These samples underwent miRNA microarray analysis using the Agilent platform. Following the preliminary screening, a larger sample set, comprising five normal prostatic tissues and 36 BPH tissues, was subjected to qRT-PCR to confirm the differential expression of the miRNAs initially identified.
The microarray analysis revealed that only miR-126-3p and miR-4672 exhibited an expression profile marked by both a fold change > 1.5 and p < 0.05, indicating significant downregulation in BPH tissues. MiR-145-3p and miR-143-3p also showed downregulation with fold changes greater than 1.5; however, these changes did not reach statistical significance as their p-values were above 0.05. Further attempts to validate these findings through qRT-PCR did not confirm any notable dysregulation among the four miRNAs studied; the variations in their expression levels between normal and BPH tissues did not achieve statistical significance, with p-values exceeding 0.1. From the data accrued, it can be inferred that the roles of miR-4672, miR-126-3p, miR-145-3p, and miR-143-3p in BPH development continue to be an unresolved mystery, and the need for further investigation.
This preliminary investigation establishes a foundation for subsequent studies aimed at elucidating the regulatory mechanisms underlying BPH. However, these results highlight the need for further investigation employing a more extensive sample size and comprehensive clinical data to elucidate their potential roles in the pathogenesis of BPH.
本研究的主要目的是识别并描述与正常前列腺组织相比，在良性前列腺增生（BPH）组织中异常表达的微小RNA（miRNA）的独特模式。
研究首先从正常前列腺组织和BPH组织中各采集三个样本。这些样本使用安捷伦平台进行miRNA微阵列分析。初步筛选后，对一个更大的样本集进行qRT-PCR，该样本集包括五个正常前列腺组织和36个BPH组织，以确认最初鉴定的miRNA的差异表达。
微阵列分析显示，只有miR-126-3p和miR-4672的表达谱具有大于1.5的倍数变化且p < 0.05，表明在BPH组织中显著下调。MiR-145-3p和miR-143-3p也显示出大于1.5的倍数变化的下调；然而，这些变化未达到统计学意义，因为它们的p值高于0.05。通过qRT-PCR进一步验证这些发现的尝试未确认所研究的四种miRNA中有任何明显的失调；它们在正常组织和BPH组织之间的表达水平变化未达到统计学意义，p值超过0.1。从积累的数据可以推断，miR-4672、miR-126-3p、miR-145-3p和miR-143-3p在BPH发展中的作用仍然是一个未解之谜，需要进一步研究。
这项初步研究为后续旨在阐明BPH潜在调控机制的研究奠定了基础。然而，这些结果凸显了需要采用更大样本量和更全面的临床数据进行进一步研究，以阐明它们在BPH发病机制中的潜在作用。
