Tabatabaie Fereshteh Hematyar, Moshtaghie Seyed Ali Asghar, Rastegari Ali Asghar, Nayeri Hashem
Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Indian J Hematol Blood Transfus. 2025 Apr;41(2):257-266. doi: 10.1007/s12288-024-01850-7. Epub 2024 Sep 19.
Iron (Fe) is one of the most abundant metallic elements on the earth and is vital for human health. Some trace elements such as cerium may interfere with iron metabolism. In the present study, the effect of cerium (Ce) interference on intestinal absorption of iron in the first stage of iron metabolism was studied. The spectrophotometry and equilibrium dialysis were used to investigate the interaction of iron and cerium. The interaction of cerium with iron metabolism by interaction with apo-transferrin was investigated. The maximum absorption wavelength of iron-transferrin and cerium-transferrin were obtained 240 nm and 465 nm, respectively. The results showed that cerium was able to bind to apo-transferrin, which binds to transferrin like saturated iron. By obtaining the iron and cerium binding constants in equilibrium dialysis (6.66 × 10 M and 3.51 × 10 M, respectively), it was determined that the site of interaction of these two ions on apo-transferrin is probably the same and cerium reduces the binding of iron to apotransferrin by 14%. Based on the results obtained in this project, it was found that cerium interferes with iron metabolism so that it can be altered through impaired intestinal absorption, plasma transport, and biochemical processes required for iron. As serum iron levels in humans are tightly regulated, the interference of cerium with iron metabolism may affect human blood functions.
铁（Fe）是地球上含量最丰富的金属元素之一，对人类健康至关重要。一些微量元素如铈可能会干扰铁代谢。在本研究中，研究了铈（Ce）干扰对铁代谢第一阶段肠道铁吸收的影响。采用分光光度法和平衡透析法研究铁与铈的相互作用。研究了铈通过与脱铁转铁蛋白相互作用对铁代谢的影响。铁转铁蛋白和铈转铁蛋白的最大吸收波长分别为240nm和465nm。结果表明，铈能够与脱铁转铁蛋白结合，其与转铁蛋白的结合方式类似于饱和铁。通过在平衡透析中获得铁和铈的结合常数（分别为6.66×10 M和3.51×10 M），确定这两种离子在脱铁转铁蛋白上的相互作用位点可能相同，铈使铁与脱铁转铁蛋白的结合减少了14%。基于本项目获得的结果，发现铈干扰铁代谢，使其可通过肠道吸收受损、血浆转运以及铁所需的生化过程而发生改变。由于人体血清铁水平受到严格调节，铈对铁代谢的干扰可能会影响人体血液功能。
