Guarino L A, Cohen S S
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3184-8. doi: 10.1073/pnas.76.7.3184.
The rate of uptake of putrescine by Anacystis nidulans has been shown to depend on the external pH and the extracellular concentration of putrescine. Accumulation of exogenous putrescine was also proportional to the concentration of putrescine in the medium, suggesting that putrescine uptake was not subject to cellular regulation. An equation was derived to test the hypothesis that putrescine accumulation was due to ion trapping. Comparison of the predicted and observed intracellular concentrations of putrescine under various conditions showed a close correlation in support of the hypothesis of ion trapping. Under conditions leading to cell death (e.g., 150 microM putrescine, pH 9.8), the correlation did not hold as a result of leakage of accumulated putrescine.
已证明集胞藻摄取腐胺的速率取决于外部pH值和腐胺的细胞外浓度。外源腐胺的积累也与培养基中腐胺的浓度成正比,这表明腐胺的摄取不受细胞调节。推导了一个方程来检验腐胺积累是由于离子捕获这一假设。比较各种条件下预测的和观察到的细胞内腐胺浓度,结果显示出密切的相关性,支持了离子捕获的假设。在导致细胞死亡的条件下(例如,150微摩尔腐胺,pH 9.8),由于积累的腐胺泄漏,这种相关性不成立。