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长链非编码RNA LINC01811吸附miR-214-3p并上调YAP1，从而促进结直肠癌的迁移和侵袭。

Long noncoding RNA LINC01811 sponges miR-214-3p and upregulates YAP1 thereby promoting the migration and invasion of colorectal cancer.

作者信息

Zhu Li, Shi Wen, Tuoheti Yiminjiang, Gong Guo-Jie, Chen Min, Liang Zong-Hua, Abudureheman Abuduweili, Gao Wei-Ge

机构信息

Department of Colorectal Surgery Ward, People's Hospital of Xinjiang Uygur Autonomous Region, No. 91 Tianchi Road, Urumqi, 830000 China.

出版信息

3 Biotech. 2025 May;15(5):123. doi: 10.1007/s13205-025-04292-8. Epub 2025 Apr 10.

DOI:10.1007/s13205-025-04292-8
PMID:40225417
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11985869/
Abstract

UNLABELLED

Long non-coding RNAs (lncRNAs) exert significant influence on the development of cancer. However, their role in colorectal cancer (CRC) is not fully clarified. The expression levels of LINC01811 in CRC samples were determined using differential expression analysis and validated by RT-qPCR assays. Transwell assays were conducted to investigate the biological function of LINC01811 in CRC. To elucidate the mechanism by which LINC01811 acts as a molecular sponge for miR-214-3p and regulates expression, binding site analysis, Luciferase reporter assay, RT-qPCR, and Western blotting were employed. We identified a novel oncogenic lncRNA LINC01811 in CRC tissues and cell lines. Our results showed that the suppression of LINC01811 significantly reduced CRC cell invasion and migration by regulating epithelial-mesenchymal transition-related markers, including MMP2, MMP9, vimentin, and E-cadherin in vitro. Furthermore, LINCO1811 modulated expression by sequestering miR-214-3p, thereby promoting CRC progression by suppressing its activity. In summary, this study identified a novel lncRNA LINC01811 involved in CRC progression through the miR-214-3p/ axis.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s13205-025-04292-8.

摘要

未标注

长链非编码RNA（lncRNAs）对癌症的发展具有重大影响。然而，它们在结直肠癌（CRC）中的作用尚未完全阐明。使用差异表达分析确定了CRC样本中LINC01811的表达水平，并通过RT-qPCR分析进行了验证。进行Transwell分析以研究LINC01811在CRC中的生物学功能。为了阐明LINC01811作为miR-214-3p的分子海绵并调节其表达的机制，采用了结合位点分析、荧光素酶报告基因检测、RT-qPCR和蛋白质免疫印迹法。我们在CRC组织和细胞系中鉴定出一种新型致癌lncRNA LINC01811。我们的结果表明，在体外，抑制LINC01811可通过调节上皮-间质转化相关标志物（包括MMP2、MMP9、波形蛋白和E-钙黏蛋白）显著降低CRC细胞的侵袭和迁移。此外，LINC01811通过螯合miR-214-3p来调节其表达，从而通过抑制其活性促进CRC进展。总之，本研究鉴定出一种新型lncRNA LINC01811，其通过miR-214-3p/轴参与CRC进展。

补充信息

在线版本包含可在10.1007/s13205-025-04292-8获取的补充材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6842/11985869/ad95bfca59fe/13205_2025_4292_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6842/11985869/9a1d4f5768b0/13205_2025_4292_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6842/11985869/dcd84461be5a/13205_2025_4292_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6842/11985869/4cc9f69e375a/13205_2025_4292_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6842/11985869/1aa64465ce84/13205_2025_4292_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6842/11985869/b1b3d78547dc/13205_2025_4292_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6842/11985869/ad95bfca59fe/13205_2025_4292_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6842/11985869/9a1d4f5768b0/13205_2025_4292_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6842/11985869/dcd84461be5a/13205_2025_4292_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6842/11985869/4cc9f69e375a/13205_2025_4292_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6842/11985869/1aa64465ce84/13205_2025_4292_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6842/11985869/b1b3d78547dc/13205_2025_4292_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6842/11985869/ad95bfca59fe/13205_2025_4292_Fig6_HTML.jpg

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