Liu Guang-Ming, Liu Yan
Department of Ophthalmology, The First People's Hospital of Changzhou, 185 Juqian Street, Tianning District, Changzhou, 213000 Jiangsu China.
Cytotechnology. 2025 Jun;77(3):88. doi: 10.1007/s10616-025-00752-4. Epub 2025 Apr 9.
Age-related macular degeneration (AMD) is a debilitating retinal disorder that may lead to progressive vision loss. One contributing factor to AMD pathogenesis is excessive blue light (BL) exposure. In this study, we investigated the therapeutic potential of exosomes derived from human umbilical cord blood mesenchymal stem cells (hUCMSC-EXs) in addressing BL-induced damage to ARPE-19 human retinal pigment epithelial (RPE) cells and explored the underlying mechanisms. Our findings revealed that BL exposure induced morphological alterations in ARPE-19 cells, accompanied by a time-dependent decline in cell viability, increased apoptosis, heightened oxidative stress, and inflammatory responses; however, hUCMSC-EXs dose-dependently mitigated BL-induced ARPE-19 cell damage. Interestingly, hUCMSC-EXs were found to suppress the upregulation of fibroblast growth factor 2 (FGF2) in BL-exposed ARPE-19 cells. Furthermore, FGF2 overexpression partially counteracted the inhibitory effects of hUCMSC-EXs on FGF2 expression and compromised the protective benefits of hUCMSC-EXs against BL-induced ARPE-19 cell damage. In conclusion, our results suggest that hUCMSC-EXs shield ARPE-19 cells from BL-induced harm by inhibiting FGF2 expression.
年龄相关性黄斑变性（AMD）是一种使人衰弱的视网膜疾病，可能导致渐进性视力丧失。AMD发病机制的一个促成因素是过度暴露于蓝光（BL）。在本研究中，我们调查了源自人脐带血间充质干细胞的外泌体（hUCMSC-EXs）在解决蓝光诱导的对ARPE-19人视网膜色素上皮（RPE）细胞损伤方面的治疗潜力，并探索了其潜在机制。我们的研究结果显示，蓝光暴露诱导了ARPE-19细胞的形态改变，同时伴随着细胞活力随时间的下降、细胞凋亡增加、氧化应激加剧以及炎症反应；然而，hUCMSC-EXs呈剂量依赖性地减轻了蓝光诱导的ARPE-19细胞损伤。有趣的是，发现hUCMSC-EXs可抑制蓝光暴露的ARPE-19细胞中成纤维细胞生长因子2（FGF2）的上调。此外，FGF2过表达部分抵消了hUCMSC-EXs对FGF2表达的抑制作用，并削弱了hUCMSC-EXs对蓝光诱导的ARPE-19细胞损伤的保护作用。总之，我们的结果表明，hUCMSC-EXs通过抑制FGF2表达保护ARPE-19细胞免受蓝光诱导的损伤。
