Zou Xiaojun, Xiao Yuan, Ke Liang, Nie Yanfeng, Xiao Jie, Yang Jinling, Guo Chaowan, Liu Xiaojuan
Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
Food Funct. 2025 May 19;16(10):3862-3878. doi: 10.1039/d5fo00291e.
For the high-value utilization of sake lees (SL), it is essential to explore its potential as a resource for anti-aging bioactives. However, the efficient extraction of SL, the compositional benefits provided, and the resulting anti-aging efficacy remain to be explored. Thus, a novel continuous phase-transition extraction (CPE) method, an amino acid analyzer, LC-MS, and GC-MS, as well as a classic anti-aging model of () were adopted. The results showed that compared to ultrasound-assisted extraction, the total amino acid content of SL extract (SLE) obtained using 80% ethanol in CPE increased by 39.64%, with a notable enhancement in the scavenging ability of free radicals ( < 0.05). In SLE, the hydrophobic, acidic, and basic amino acids with antioxidant activity accounted for 77.11% of total amino acids. New potential anti-aging compounds were identified, including Lys-Gln, Leu-Arg-Lys, and sphinganine. In particular, 4 mg mL SLE not only promoted a 19.32% increase in the lifespan of by enhancing oxidative stress and neuroprotective effects but also ameliorated age-related phenotypes like motoricity and age pigment. Further exploration revealed that the efficacy of SLE is mediated by SKN-1/Nrf2 and HSF-1 pathways, which can be confirmed by the upregulation of key genes, such as and , especially by inducing a 72.73% increase in nuclear transfer of the transcription factor SKN-1/Nrf2. Taken together, SLE obtained by CPE was abundant in bioactives and contains novel components, thus exerting prominent anti-aging effects . This study provides a new way to obtain anti-aging active substances efficiently, which is beneficial for application in the fields of health foods and cosmetics.
为了实现酒糟（SL）的高值化利用，探索其作为抗衰老生物活性物质资源的潜力至关重要。然而，SL的高效提取、所提供的成分益处以及由此产生的抗衰老功效仍有待探索。因此，采用了一种新型的连续相转变提取（CPE）方法、氨基酸分析仪、液相色谱 - 质谱联用仪和气相色谱 - 质谱联用仪，以及经典的（）抗衰老模型。结果表明，与超声辅助提取相比，在CPE中使用80%乙醇获得的SL提取物（SLE）的总氨基酸含量增加了39.64%，自由基清除能力显著增强（<0.05）。在SLE中，具有抗氧化活性的疏水、酸性和碱性氨基酸占总氨基酸的77.11%。鉴定出了新的潜在抗衰老化合物，包括Lys - Gln、Leu - Arg - Lys和鞘氨醇。特别是，4mg/mL的SLE不仅通过增强氧化应激和神经保护作用使（）的寿命延长了19.32%，还改善了与年龄相关的表型，如运动能力和老年色素。进一步研究发现，SLE的功效是由SKN - 1/Nrf2和HSF - 1途径介导的，这可以通过关键基因（如（）和（））的上调得到证实，特别是通过诱导转录因子SKN - 1/Nrf2的核转位增加72.73%得到证实。综上所述，通过CPE获得的SLE富含生物活性物质且含有新成分，从而发挥出显著的抗衰老作用。本研究为高效获得抗衰老活性物质提供了一种新途径，有利于在保健食品和化妆品领域的应用。
