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通过对流免疫电泳检测粪便中的蓝氏贾第鞭毛虫抗原。

Detection of Giardia lamblia antigen in the feces by counterimmunoelectrophoresis.

作者信息

Vinayak V K, Kum K, Chandna R, Venkateswarlu K, Mehta S

出版信息

Pediatr Infect Dis. 1985 Jul-Aug;4(4):383-6. doi: 10.1097/00006454-198507000-00010.

DOI:10.1097/00006454-198507000-00010
PMID:4022804
Abstract

The presence of Giardia lamblia antigen in stools of symptomatic and asymptomatic giardiasis patients was studied by counterimmunoelectrophoresis using anti-G. lamblia (Portland strain I) trophozoite antibodies. This test could detect antigen in feces of 94% of giardiasis patients. However, it failed to differentiate between symptomatic and asymptomatic Giardia infection. There were no false-positive results in children with other diseases. Antigen in the stools disappeared within 1 to 2 weeks after eradication of infection with specific treatment. The overall sensitivity of this test in detecting G. lamblia antigen in stool was 94%, and the specificity was 95%.

摘要

采用抗蓝氏贾第鞭毛虫(波特兰I株)滋养体抗体,通过对流免疫电泳法研究了有症状和无症状贾第虫病患者粪便中蓝氏贾第鞭毛虫抗原的存在情况。该检测方法可在94%的贾第虫病患者粪便中检测到抗原。然而,它无法区分有症状和无症状的贾第虫感染。其他疾病患儿未出现假阳性结果。经特异性治疗根除感染后,粪便中的抗原在1至2周内消失。该检测方法检测粪便中蓝氏贾第鞭毛虫抗原的总体敏感性为94%,特异性为95%。

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