用于检测蓝氏贾第鞭毛虫的酶免疫测定法。
Enzyme immunoassay for the detection of Giardia lamblia.
作者信息
Carlson J R, Sullivan P S, Harry D J, Stork M A, Thorton S A, DuPont H L
机构信息
Department of Pathology, School of Medicine, University of California, Davis 95616.
出版信息
Eur J Clin Microbiol Infect Dis. 1988 Aug;7(4):538-40. doi: 10.1007/BF01962610.
An enzyme immunoassay (EIA) was developed for direct detection of Giardia lamblia antigens in fecal specimens. The EIA was evaluated by testing specimens from 1,331 subjects in the USA and Egypt. For the 353 specimens from human subjects in the USA there was a 97% overall agreement between the results of the EIA and direct microscopic examination, yielding a sensitivity and specificity of 92% and 99% respectively. Due to adverse field conditions the EIA did not perform as well in the specimens collected and tested in Egypt. The sensitivity and specificity for 585 human specimens from Egypt were 74% and 97% respectively.
已开发出一种酶免疫测定法（EIA），用于直接检测粪便标本中的蓝氏贾第鞭毛虫抗原。通过检测来自美国和埃及的1331名受试者的标本对该EIA进行了评估。对于来自美国人体受试者的353份标本，EIA结果与直接显微镜检查结果的总体一致性为97%，灵敏度和特异性分别为92%和99%。由于野外条件恶劣，EIA在埃及收集和检测的标本中表现不佳。来自埃及的585份人体标本的灵敏度和特异性分别为74%和97%。
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