He Yiyao, He Dawei, Ren Song, Fan Lin, Wang Lin, Sun Jiang
Department of Stomatology, Mianyang Hospital of TCM, Mianyang, China.
Department of Periodontics and Oral Mucosa Diease, Dalian Stomatological Hospital, Dalian, China.
Int J Pharm. 2025 May 15;676:125569. doi: 10.1016/j.ijpharm.2025.125569. Epub 2025 Apr 12.
Gingival recession is a common clinical concern. While surgical intervention remains the conventional approach for periodontal soft tissue regeneration, it is often associated with trauma. Recent advancements emphasize minimally invasive and effective alternatives. This study developed a hydrogel microneedle (MN) patch loaded with madecassoside using 3D printing technology to promote periodontal soft tissue regeneration. The PEGDA hydrogel-based MN patch exhibited excellent mechanical properties and biocompatibility, enabling effective skin penetration. In vitro studies demonstrated that madecassoside at specific concentrations enhanced gingival fibroblast proliferation and type I collagen expression. Animal experiments further confirmed that microneedles containing madecassoside effectively promoted periodontal soft tissue regeneration in rabbits. These findings demonstrate the potential of 3D-printed hydrogel microneedles as a promising approach for periodontal soft tissue regeneration, supporting both tissue repair and collagen synthesis.
牙龈退缩是一种常见的临床问题。虽然手术干预仍然是牙周软组织再生的传统方法，但它常常伴随着创伤。最近的进展强调微创且有效的替代方法。本研究利用3D打印技术开发了一种负载积雪草苷的水凝胶微针（MN）贴片，以促进牙周软组织再生。基于聚乙二醇二丙烯酸酯（PEGDA）水凝胶的MN贴片表现出优异的机械性能和生物相容性，能够有效穿透皮肤。体外研究表明，特定浓度的积雪草苷可增强牙龈成纤维细胞增殖和I型胶原蛋白表达。动物实验进一步证实，含有积雪草苷的微针可有效促进兔牙周软组织再生。这些发现证明了3D打印水凝胶微针作为牙周软组织再生的一种有前景的方法的潜力，对组织修复和胶原蛋白合成均有支持作用。
