Wang Guanglong, Xu Yujie, Wu Jiaqi, Chen Yangyang, An Yahong, Hu Zhenzhu, Xiong Aisheng
School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China.
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
BMC Plant Biol. 2025 Apr 15;25(1):470. doi: 10.1186/s12870-025-06497-8.
Carrot is a root vegetable abundant in numerous nutritional values. Sugar is one of the most important carbohydrates in horticultural products that play important roles in plant growth and development and response to biotic and abiotic stresses. However, the dynamics of the metabolites including sugar during carrot root development still remain unclear. Here, the differential metabolites in carrot roots at different developmental stages were measured using an UPLC-ESI-MS/MS system. The accumulation profiles of metabolites, especially sugars, as well as the transcript patterns of Sugars Will Eventually be Exported Transporter (SWEET) genes were intensively examined.
The results identified 727 metabolites over all the samples detected, of which, 539 metabolites were found to be differential accumulated. A total of 34 differentially accumulated sugar metabolites were identified over the period of root development. Furthermore, 17 DcSWEET genes were detected to be specifically expressed in the roots, indicating a potential for root enlargement and sugar accumulation in carrot root.
The results from the current study would help carrot breeding focused on yield and quality improvement.
胡萝卜是一种富含多种营养价值的根菜类蔬菜。糖类是园艺产品中最重要的碳水化合物之一，在植物生长发育以及对生物和非生物胁迫的响应中发挥着重要作用。然而，胡萝卜根发育过程中包括糖类在内的代谢物动态仍不清楚。在此，使用超高效液相色谱-电喷雾串联质谱（UPLC-ESI-MS/MS）系统测定了胡萝卜根在不同发育阶段的差异代谢物。深入研究了代谢物尤其是糖类的积累谱，以及糖输出转运蛋白（SWEET）基因的转录模式。
在所有检测样品中鉴定出727种代谢物，其中539种代谢物被发现差异积累。在根发育期间共鉴定出34种差异积累的糖类代谢物。此外，检测到17个DcSWEET基因在根中特异性表达，表明胡萝卜根具有膨大及糖分积累的潜力。
本研究结果将有助于以提高产量和品质为重点的胡萝卜育种。
