Sarioğlu Ali
Department of Soil Science and Plant Nutrition, Harran University, Sanliurfa, Turkey.
BMC Plant Biol. 2025 Apr 15;25(1):473. doi: 10.1186/s12870-025-06477-y.
This study aimed to evaluate the effects of potassium (K) and Bradyrhizobium japonicum applications on physiological and microbial parameters in soybean plants under salt stress. The study included treatments of control, potassium (2.2 g KSO), bacteria (B), and their combinations (K + B), along with versions exposed to 100 mM NaCl salt stress. Key parameters such as leaf water content (RWC), chlorophyll (SPAD, Chlo a/b), oxidative stress indicators (HO and MDA), proline, protein, antioxidant enzyme activities (APX, POD, and CAT), microbial biomass carbon (MBC), and CO release from soil were measured. Salt stress reduced RWC in plants by 15%, while HO and MDA levels increased by 25% and 30%, respectively. However, potassium and bacterial applications improved plant resilience against stress by increasing proline levels by 20%, reducing protein loss by 18%, and enhancing antioxidant enzyme activities to mitigate oxidative damage. In soil microbial activities, MBC increased by up to 161%, and CO₂ release increased by up to 27.7% with K + B application. Under salt stress, MBC and CO₂ release were restored by 122% and 50.8%, respectively, demonstrating the positive effects of potassium and bacterial inoculation on microbial activity. These findings suggest that potassium and Bradyrhizobium japonicum applications could be considered effective strategies for enhancing plant tolerance and soil health under salt stress conditions.
本研究旨在评估盐胁迫下施用钾（K）和日本慢生根瘤菌对大豆植株生理和微生物参数的影响。该研究包括对照、钾（2.2 g KSO）、细菌（B）及其组合（K + B）处理，以及暴露于100 mM NaCl盐胁迫的处理。测量了关键参数，如叶片含水量（RWC）、叶绿素（SPAD、Chlo a/b）、氧化应激指标（HO和MDA）、脯氨酸、蛋白质、抗氧化酶活性（APX、POD和CAT）、微生物生物量碳（MBC）以及土壤中CO₂的释放量。盐胁迫使植物的RWC降低了15%，而HO和MDA水平分别增加了25%和30%。然而，施用钾和细菌通过使脯氨酸水平提高20%、减少18%的蛋白质损失以及增强抗氧化酶活性以减轻氧化损伤，从而提高了植物的抗逆能力。在土壤微生物活性方面，施用K + B时，MBC增加了高达161%，CO₂释放量增加了高达27.7%。在盐胁迫下，MBC和CO₂释放量分别恢复了122%和50.8%，这表明钾和细菌接种对微生物活性具有积极影响。这些发现表明，在盐胁迫条件下，施用钾和日本慢生根瘤菌可被视为增强植物耐受性和土壤健康的有效策略。
