Man R Y, Lederman C L
Pharmacology. 1985;31(1):11-6. doi: 10.1159/000138092.
Lysophosphatidylcholine (LPC) had recently been shown to accumulate in the ischemic heart and was demonstrated to be arrhythmogenic in perfused hearts. The present study was designed to examine the effects of calcium and verapamil on LPC-induced arrhythmias. Rat hearts were perfused through the aorta with oxygenated Krebs-Henseleit buffer at 37 degrees C. The occurrence of ventricular fibrillation was dependent on the LPC concentration and perfusion time. In subsequent experiments, each heart was perfused with a buffer containing 20 microM LPC for 3 min and was followed with a 10 min washout period. Ventricular fibrillation occurred in all 8 control experiments. When the Ca2+ concentration was reduced to half, only 3 out of 8 hearts fibrillated. In the presence of 0.2 mg/1 of verapamil, 7 out of 9 hearts fibrillated, and the time to fibrillation was not increased significantly by verapamil. These results suggest that calcium may be important in LPC-induced arrhythmias, and this effect is not significantly attenuated by the Ca2+ antagonist, verapamil.
溶血磷脂酰胆碱（LPC）最近被证明会在缺血心脏中蓄积，并且在灌注心脏中被证实具有致心律失常作用。本研究旨在探讨钙和维拉帕米对LPC诱导的心律失常的影响。大鼠心脏在37℃下通过主动脉用含氧的克雷布斯 - 亨泽莱特缓冲液灌注。室颤的发生取决于LPC浓度和灌注时间。在随后的实验中，每颗心脏用含有20μM LPC的缓冲液灌注3分钟，然后有10分钟的洗脱期。在所有8个对照实验中均发生了室颤。当Ca2 +浓度降至一半时，8颗心脏中只有3颗发生了颤动。在存在0.2mg/1维拉帕米的情况下，9颗心脏中有7颗发生了颤动，维拉帕米并未显著延长发生颤动的时间。这些结果表明，钙在LPC诱导的心律失常中可能起重要作用，并且这种作用不会被Ca2 +拮抗剂维拉帕米显著减弱。
