Sousa Mauricio G C, Brasino Danielle S K, Krieger Madeline, Dindar Duygu A, Duhen Rebekka, Zhang Zhenzhen, Franca Cristiane Miranda, Bertassoni Luiz E
Knight Cancer Precision Biofabrication Hub, Knight Cancer Institute, Oregon Health & Science University, Portland, OR, United States.
Cancer Early Detection Advanced Research Center, Oregon Health & Science University, Portland, OR, United States.
Front Bioeng Biotechnol. 2025 Mar 31;13:1505963. doi: 10.3389/fbioe.2025.1505963. eCollection 2025.
The tumor microbiota has emerged as a pivotal contributor to a variety of cancers, impacting disease development, progression, and therapeutic resistance. Due to the complexity of the tumor microenvironment, reproducing the interactions between the microbes, tumor cells, and the immune system remains a great challenge for both and studies. To this end, significant progress has been made toward leveraging tumor-on-a-chip model systems to replicate critical hallmarks of the native disease . These microfluidic platforms offer the ability to mimic essential components of the tumor microenvironment, including controllable fluid flow conditions, manipulable extracellular matrix dynamics, and intricate 3D multi-cellular communication. The primary objective of this review is to discuss recent challenges and advances in engineering host-microbiota and tumor interactions on-a-chip. Ultimately, overcoming these obstacles will help us gain deeper insights into tumor-microbe interactions and enhance avenues for developing more effective cancer therapies.
肿瘤微生物群已成为多种癌症的关键促成因素，影响疾病的发生、发展和治疗抗性。由于肿瘤微环境的复杂性，再现微生物、肿瘤细胞和免疫系统之间的相互作用对体内和体外研究来说仍然是一个巨大的挑战。为此，在利用芯片上肿瘤模型系统来复制天然疾病的关键特征方面已经取得了重大进展。这些微流控平台能够模拟肿瘤微环境的基本组成部分，包括可控的流体流动条件、可操纵的细胞外基质动态以及复杂的三维多细胞通讯。本综述的主要目的是讨论在芯片上构建宿主-微生物群与肿瘤相互作用方面的最新挑战和进展。最终，克服这些障碍将有助于我们更深入地了解肿瘤-微生物相互作用，并增加开发更有效癌症治疗方法的途径。
