RNA去帽蛋白GhLSM1BS的鉴定与过表达：通过上调油菜素甾醇生物合成增强棉花体细胞胚胎发生
Identification and overexpression of RNA-decapping protein GhLSM1BS: Enhancing cotton somatic embryogenesis through up-regulating brassinosteroid biosynthesis.
作者信息
Yang Zheng, He Jie, Yao Shuqian, Li Xuxin, Du Chang, Gong Juwu, Yan Haoliang, Zhao Yanpeng, Li Yang, Yuan Youlu, Shang Haihong
机构信息
Zhengzhou Research Base, National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, China.
National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
出版信息
Plant Biotechnol J. 2025 Jul;23(7):2670-2672. doi: 10.1111/pbi.70090. Epub 2025 Apr 15.
We identified two splicing variants of GhLSM1B (GhLSM1BS and GhLSM1BL) with distinct expression patterns and predicted 3D structures, despite sharing the same nuclear localization. Overexpression of GhLSM1BS, but not GhLSM1BL, accelerated cotton callus proliferation and altered cell morphology during somatic embryogenesis, accompanied by altered expression of CYP450 family genes and elevated brassinosteroid levels.
我们鉴定出了陆地棉LSM1B（GhLSM1B）的两种剪接变体（GhLSM1BS和GhLSM1BL），尽管它们具有相同的核定位，但却有着不同的表达模式和预测的三维结构。GhLSM1BS的过表达而非GhLSM1BL的过表达加速了棉花愈伤组织的增殖，并在体细胞胚胎发生过程中改变了细胞形态，同时伴随着细胞色素P450家族基因表达的改变和油菜素类固醇水平的升高。
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