State Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450000, China.
Sci China Life Sci. 2018 Dec;61(12):1566-1582. doi: 10.1007/s11427-018-9412-x. Epub 2018 Nov 26.
Brassinosteroids (BRs), which are essential phytohormones for plant growth and development, are important for cotton fiber development. Additionally, BES1 transcription factors are critical for BR signal transduction. However, cotton BES1 family genes have not been comprehensively characterized. In this study, we identified 11 BES1 genes in G. arboreum, 11 in G. raimondii, 16 in G. barbadense, and 22 in G. hirsutum. The BES1 sequences were significantly conserved in the Arabidopsis thaliana, rice, and upland cotton genomes. A total of 94 BES1 genes from 10 different plant species were divided into three clades according to the neighbor-joining and minimum-evolution methods. Moreover, the exon/intron patterns and motif distributions were highly conserved among the A. thaliana and cotton BES1 genes. The collinearity among the orthologs from the At and Dt subgenomes was estimated. Segmental duplications in the At and Dt subgenomes were primarily responsible for the expansion of the cotton BES1 gene family. Of the GhBES1 genes, GhBES1.4_At/Dt exhibited BL-induced expression and was predominantly expressed in fibers. Furthermore, Col-0/mGhBES1.4_At plants produced curled leaves with long and bent petioles. These transgenic plants also exhibited decreased hypocotyl sensitivity to brassinazole and constitutive BR induced/repressed gene expression patterns. The constitutive BR responses of the plants overexpressing mGhBES1.4_At were similar to those of the bes1-D mutant.
油菜素内酯（BRs）是植物生长发育所必需的植物激素，对棉花纤维发育很重要。此外，BES1 转录因子对于 BR 信号转导至关重要。然而，棉花 BES1 家族基因尚未得到全面的描述。在本研究中，我们在陆地棉中鉴定到了 11 个 BES1 基因，在雷蒙德氏棉中鉴定到了 11 个，在海岛棉中鉴定到了 16 个，在亚洲棉中鉴定到了 22 个。BES1 序列在拟南芥、水稻和陆地棉基因组中高度保守。根据邻接法和最小进化法，将来自 10 种不同植物的总共 94 个 BES1 基因分为三个分支。此外，拟南芥和棉花 BES1 基因的外显子/内含子模式和基序分布高度保守。对 At 和 Dt 亚基因组的同源基因进行了共线性估计。At 和 Dt 亚基因组的片段重复是棉花 BES1 基因家族扩张的主要原因。在 GhBES1 基因中，GhBES1.4_At/Dt 表现出 BL 诱导表达，主要在纤维中表达。此外，Col-0/mGhBES1.4_At 植株产生卷曲的叶片，叶柄长而弯曲。这些转基因植株还表现出对油菜素唑的下胚轴敏感性降低和组成型 BR 诱导/抑制基因表达模式。过表达 mGhBES1.4_At 的植物表现出与 bes1-D 突变体相似的组成型 BR 响应。
