从枯草芽孢杆菌中分离出的一种膜 - 脱氧核糖核酸复合物的某些特性。
Some properties of a membrane-deoxyribonucleic acid complex isolated from Bacillus subtilis.
作者信息
Harmon J M, Taber H W
出版信息
J Bacteriol. 1977 Feb;129(2):789-95. doi: 10.1128/jb.129.2.789-795.1977.
Abstract
Membrane-deoxyribonucleic acid (DNA) complexes were isolated from Bacillus subtilis by affinity for magnesium-Sarkosyl crystals. These complexes (M-bands) contained greater than 80% of the total cellular DNA; little of the remaining portion could be recovered in a secondary isolation. Isotopic labeling of the origin of replication showed this region of the chromosome to be closely associated with the cell membrane. Interruption of protein or DNA synthesis did not result in detachment of the chromosome from the membrane. Interruption of ribonucleic acid synthesis by rifampin resulted in a decreased ability to isolate DNA in the M-band. Analysis of attachment of the chromosome to membrane during the cell and replication cycles indicated that the chromosome is not released from the membrane at any time during the cell cycle.
摘要
通过对镁-十二烷基肌氨酸钠晶体的亲和力从枯草芽孢杆菌中分离出膜-脱氧核糖核酸（DNA）复合物。这些复合物（M带）包含了细胞总DNA的80%以上；其余部分很少能在二次分离中回收。复制起点的同位素标记表明染色体的这个区域与细胞膜紧密相关。蛋白质或DNA合成的中断不会导致染色体从膜上脱离。利福平对核糖核酸合成的中断导致在M带中分离DNA的能力下降。对细胞和复制周期中染色体与膜的附着分析表明，在细胞周期的任何时候染色体都不会从膜上释放。
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