Beeson J, Sueoka N
J Bacteriol. 1979 Sep;139(3):911-6. doi: 10.1128/jb.139.3.911-916.1979.
A temperature-sensitive Bacillus subtilis initiation mutant was used to achieve one cycle of synchronized deoxyribonucleic acid (DNA) replication. Markers near the origin of replication and the terminus were assayed for association with the cell membrane at intervals during the DNA replication cycle. DNA near the origin and terminus was found to be enriched in the membrane fraction throughout the DNA replication cycle. The magnitude of membrane enrichment or origin and terminus markers varied coincidentally, possibly as a consequence of incubating the cells at 45 degrees C.
利用一种温度敏感型枯草芽孢杆菌起始突变体实现了一轮同步的脱氧核糖核酸(DNA)复制。在DNA复制周期中,每隔一段时间检测复制起点和终点附近的标记物与细胞膜的关联情况。发现在整个DNA复制周期中,复制起点和终点附近的DNA在膜组分中富集。膜富集程度以及起点和终点标记物的变化是同步的,这可能是将细胞在45摄氏度下培养的结果。