Holmberg J T, Hederström E, Ihse I
Scand J Gastroenterol. 1985 May;20(4):428-32. doi: 10.3109/00365528509089675.
The purpose of this study was to develop a surgical technique for bile duct occlusion in the rat which would prevent restoration of biliary drainage. In three successive series of animals the surgical technique was modified from ligation and transection to ligation and transection with resection and, finally, to ligation and transection with transposition of the distal end behind the stomach-duodenum. In these three groups recanalization was observed in 4 out of 9, 1 out of 9, and 0 out of 19 animals, respectively. Bilirubin levels in plasma monitored in the last group showed that a stable cholestatic jaundice was maintained for at least 6 weeks. The results suggest that transposition of the distal transected end of the bile duct below the stomach-duodenum will effectively reduce the chance of recanalization and thus make study of long-term extrahepatic cholestasis in the rat more practical.
本研究的目的是开发一种用于大鼠胆管闭塞的手术技术，该技术可防止胆汁引流恢复。在连续三组动物中，手术技术从结扎和横断改为结扎、横断并切除，最后改为结扎、横断并将远端置于胃十二指肠后方。在这三组中，分别有9只动物中的4只、9只动物中的1只和19只动物中的0只观察到再通。在最后一组中监测的血浆胆红素水平表明，稳定的胆汁淤积性黄疸至少维持了6周。结果表明，将胆管横断的远端置于胃十二指肠下方可有效降低再通的几率，从而使大鼠长期肝外胆汁淤积的研究更具可行性。
