Ivancevic Atma, Sankovitz Madison, Allen Holly, Joyner Olivia, Chuong Edward B, Ramsey Samuel D
bioRxiv. 2025 Apr 6:2025.04.01.646657. doi: 10.1101/2025.04.01.646657.
The subgenus, which includes the black dwarf honey bee ( ) and the red dwarf honey bee ( ), remains underrepresented in genomic studies despite its ecological significance. Here, we present high-quality de novo genome assemblies for both species, generated using a hybrid sequencing approach combining Oxford Nanopore Technologies (ONT) long reads with Illumina short reads. The final assemblies are highly contiguous, with contig N50 values of 5.0 Mb ( ) and 4.3 Mb ( ), representing a major improvement over the previously published genome. Genome completeness assessments indicate high quality, with BUSCO scores exceeding 98.5% and k-mer analyses supporting base-level accuracy. Repeat annotation revealed a relatively low repetitive sequence content (∼6%), consistent with other species. Using RNA sequencing data, we annotated 12,232 genes for and 12,597 genes for , with >97% completeness in predicted proteomes. These genome assemblies provide a valuable resource for comparative and functional genomic studies, offering new insights into the genetic basis of dwarf honey bee adaptations.
该亚属包括黑小蜜蜂（ ）和红小蜜蜂（ ），尽管具有生态意义，但在基因组研究中的代表性仍然不足。在此，我们展示了这两个物种的高质量从头基因组组装结果，采用了将牛津纳米孔技术（ONT）长读长与Illumina短读长相结合的混合测序方法生成。最终的组装结果具有高度的连续性，重叠群N50值分别为5.0 Mb（ ）和4.3 Mb（ ），相较于之前发表的基因组有了显著改进。基因组完整性评估表明质量很高，BUSCO得分超过98.5%，k-mer分析支持碱基水平的准确性。重复序列注释显示重复序列含量相对较低（约6%），与其他 物种一致。利用RNA测序数据，我们为 注释了12232个基因，为 注释了12597个基因，预测蛋白质组的完整性超过97%。这些基因组组装为比较和功能基因组研究提供了宝贵资源，为小蜜蜂适应的遗传基础提供了新的见解。
