通过抑制结构剪接增强子对……进行自动调节。 （原文“Autoregulation of by...”表述不完整，这里按正常逻辑补充完整表述进行翻译）
Autoregulation of by inhibition of a structural splicing enhancer.
作者信息
Granas David, Hewa Ishan Gammadde, White Michael A, Stormo Gary D
出版信息
bioRxiv. 2025 Apr 2:2025.03.14.643126. doi: 10.1101/2025.03.14.643126.
Yeast ribosomal protein gene is autoregulated by inhibition of splicing. The first intron has a "zipper stem" that brings the 5' splice site near the branch point and serves as an enhancer of splicing that is required for efficient splicing because it has non-consensus branch point sequence of UGCUAAC. The intron also contains an alternative, and mutually exclusive, structure that is conserved across many yeast species. That conserved structure is a binding site for the Rpl7 protein so that when the protein is in excess over what is required for ribosomes, the protein binds to the conserved structure which eliminates the enhancer structure and represses splicing and gene expression.
酵母核糖体蛋白基因通过剪接抑制进行自身调节。第一个内含子有一个“拉链茎”，它将5'剪接位点带到分支点附近，并作为剪接增强子，由于其具有非共识的UGCUAAC分支点序列，所以对有效剪接是必需的。该内含子还包含一种在许多酵母物种中保守的替代且相互排斥的结构。该保守结构是Rpl7蛋白的结合位点，因此当该蛋白超过核糖体所需量时，它会与保守结构结合，从而消除增强子结构并抑制剪接和基因表达。
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