Saiedullah Muhammad, Nila Nurun Nahar, Mahmud Zimam, Tamanna Sonia, Hassan Md Zahid, Hossain Howlader Md Zakir
Laboratory of Nutrition and Health Research, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh.
Department of Physiology & Molecular Biology, Bangladesh University of Health Sciences (BUHS), Dhaka, Bangladesh.
Biochem Biophys Rep. 2025 Apr 3;42:101992. doi: 10.1016/j.bbrep.2025.101992. eCollection 2025 Jun.
Cholesteryl ester transfer protein (CETP) gene polymorphisms influence CETP expression and high-density lipoprotein cholesterol (HDL-c) levels, yet their genetic impact remains unexplored in the Bangladeshi population, where low HDL-c is prevalent. This study examined the association of CETP -629C/A and 277C/T polymorphisms with circulating HDL-c levels in 402 individuals (217 males, 185 females). Serum lipid profiles were measured using an automated analyzer, and CETP polymorphisms were genotyped using PCR-RFLP. The -629C/A and 277C/T polymorphisms were in Hardy-Weinberg equilibrium, with heterozygous genotypes being the most frequent. While -629C/A genotypes showed no significant difference between the high and low HDL-c groups, individuals carrying the -629AA and CA + AA genotypes had significantly higher HDL-c levels compared to CC carriers (p = 0.023, p = 0.043). For the 277C/T, TT genotype differed significantly between the high and low HDL-c groups (p = 0.011, OR = 0.37) and, individuals carrying the 277 TT and CT + TT genotypes had significantly higher HDL-c compared to the CC genotype (p = 0.002, p = 0.019). Additionally, allelic analysis suggested a marginal association between the 277T allele and increased HDL-c levels (p = 0.051, OR = 0.59). Multiple regression analysis confirmed an inverse association between -629CC (β = -1.106, p = 0.038) and 277CC + CT (β = -0.963, p = 0.016) with HDL-c levels. However, no significant differences were observed in total cholesterol, triglycerides, LDL-c, or apolipoprotein levels across genotypes. These findings suggest that CETP -629CC, 277CC, and CT genotypes contribute to low HDL-c levels in the Bangladeshi population, highlighting the potential role of CETP genetic screening as a biomarker for identifying individuals at risk of HDL-c deficiency and associated cardiovascular complications.
胆固醇酯转运蛋白（CETP）基因多态性会影响CETP表达和高密度脂蛋白胆固醇（HDL-c）水平，然而在HDL-c水平较低的孟加拉人群体中，其遗传影响仍未得到探索。本研究检测了402名个体（217名男性，185名女性）中CETP -629C/A和277C/T多态性与循环HDL-c水平之间的关联。使用自动分析仪测量血清脂质谱，并采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对CETP多态性进行基因分型。-629C/A和277C/T多态性处于哈迪-温伯格平衡，杂合基因型最为常见。虽然-629C/A基因型在高HDL-c组和低HDL-c组之间无显著差异，但携带-629AA和CA + AA基因型的个体相比CC携带者具有显著更高的HDL-c水平（p = 0.023，p = 0.043）。对于277C/T，TT基因型在高HDL-c组和低HDL-c组之间存在显著差异（p = 0.011，比值比[OR]=0.37），并且携带277 TT和CT + TT基因型的个体相比CC基因型具有显著更高的HDL-c水平（p = 0.002，p = 0.019）。此外，等位基因分析表明277T等位基因与HDL-c水平升高之间存在边缘关联（p = 0.051，OR = 0.59）。多元回归分析证实-629CC（β=-1.106，p = 0.038）和277CC + CT（β=-0.963，p = 0.016）与HDL-c水平呈负相关。然而，不同基因型在总胆固醇、甘油三酯、低密度脂蛋白胆固醇或载脂蛋白水平上未观察到显著差异。这些发现表明，CETP -629CC、277CC和CT基因型导致了孟加拉人群体中HDL-c水平较低，突出了CETP基因筛查作为一种生物标志物在识别HDL-c缺乏风险个体及相关心血管并发症方面的潜在作用。
