OBJECTIVE: Myofascial pain syndrome (MPS) is a common condition. Manual therapy is preferred over other invasive treatments due to its noninvasiveness and safety, but evidence for its effectiveness is lacking. We here conducted a comprehensive search of randomized controlled trial studies related to manipulative therapy for MPS and conducted a network meta-analysis to provide a basis for clinical applications and experimental studies. METHODS: Reports on randomized controlled trials of noninvasive therapy for MPS deposited in the China National Knowledge Infrastructure database, Chinese Science Citation Database(CSCD), Chinese Citation Database(CCD), Chinese Biomedical Database, PubMed, Embase, Cochrane Library, and Web of Science from their respective inception dates until November 10, 2024, were retrieved. A network meta-analysis was performed using Rv4.2.0 software. RESULTS: Fourteen studies involving 588 patients overall were included. The interventions included pain point compression, massage, and ischemic compression. Visual analogue scale scores were reported in 12 studies and the pressure pain threshold was reported in five studies. The outcomes demonstrated that manipulation had a notable effect, although not statistically significant, as compared with conventional treatment and placebo. Overall, the effect of pain-point compression was favorable. The results were stable, with no significant publication bias. CONCLUSION: Manipulation was an effective treatment for MPS, but the effects were not statistically significant, based on the findings of this study. Owing to issues such as uneven methodological quality and the small sample size of the included studies, further high-quality multicenter, large-sample, randomized double-blind controlled trials are required to verify the reliability of this conclusion.