在实时定量聚合酶链反应（RT-qPCR）检测的模拟运输环境条件下，评估[具体物质]在各种介质中的稳定性。
Evaluating the stability of in various media under simulated environmental conditions of shipment for RT-qPCR assays.
作者信息
Boggan SaraBeth, Williams Ryan B, Koziol Jennifer H
机构信息
Texas Tech School of Veterinary Medicine, Amarillo, TX, USA.
出版信息
J Vet Diagn Invest. 2025 Apr 16:10406387251330297. doi: 10.1177/10406387251330297.
Abstract
is a notable reproductive pathogen in cattle, and the processes of sample collection, handling, transportation, and testing present considerable challenges for surveillance initiatives. Advancements include the development of a direct detection method for using reverse-transcription quantitative real-time PCR. We compared the stability of samples in 3 different media: PBS, sterile saline, and lactated Ringer solution over periods of 24-120 h, with storage temperatures of 0°C, 20°C, 37°C, and 54.5°C to mimic shipping of samples in various environmental temperatures.
摘要
是牛身上一种显著的生殖病原体，样本采集、处理、运输和检测过程给监测工作带来了巨大挑战。进展包括开发了一种使用逆转录定量实时PCR的直接检测方法。我们比较了样本在3种不同培养基（磷酸盐缓冲液、无菌生理盐水和乳酸林格氏液）中在24 - 120小时内的稳定性，储存温度为0°C、20°C、37°C和54.5°C，以模拟在各种环境温度下样本的运输情况。
相似文献
1
Evaluating the stability of in various media under simulated environmental conditions of shipment for RT-qPCR assays.在实时定量聚合酶链反应（RT-qPCR）检测的模拟运输环境条件下，评估[具体物质]在各种介质中的稳定性。
J Vet Diagn Invest. 2025 Apr 16:10406387251330297. doi: 10.1177/10406387251330297.
2
Effects of bacterial contamination of media on the diagnosis of Tritrichomonas foetus by culture and real-time PCR.培养基细菌污染对通过培养和实时荧光定量PCR诊断胎儿三毛滴虫的影响。
Vet Parasitol. 2015 Mar 15;208(3-4):143-9. doi: 10.1016/j.vetpar.2015.01.006. Epub 2015 Jan 19.
3
Pooling of cultured samples and comparison of multistate laboratory workflows with the MagMAX sample preparation system and VetMAX quantitative polymerase chain reaction reagents for detection of Tritrichomonas foetus-colonized bulls.将培养样本合并，并比较使用MagMAX样本制备系统和VetMAX定量聚合酶链反应试剂的多状态实验室工作流程，以检测感染胎儿三毛滴虫的公牛。
J Vet Diagn Invest. 2014 Jan;26(1):72-87. doi: 10.1177/1040638713510003. Epub 2013 Dec 16.
4
The influence of temperature and simulated transport conditions of diagnostic samples on real-time polymerase chain reaction for the detection of Tritrichomonas foetus DNA.诊断样本的温度和模拟运输条件对检测胎儿三毛滴虫DNA的实时聚合酶链反应的影响。
J Vet Diagn Invest. 2011 Sep;23(5):982-5. doi: 10.1177/1040638711417143.
5
Improvements in Tritrichomonas foetus molecular testing.胎儿三毛滴虫分子检测的改进
J Vet Diagn Invest. 2018 Jul;30(4):603-608. doi: 10.1177/1040638718767943. Epub 2018 Apr 10.
6
Evaluation of effects of high incubation temperatures on results of protozoal culture and real-time PCR testing for Tritrichomonas foetus inoculated in a commercially available self-contained culture media system.评估高温孵育对接种于市售独立培养基系统中的胎儿三毛滴虫原虫培养结果及实时聚合酶链反应检测结果的影响。
J Am Vet Med Assoc. 2011 Dec 15;239(12):1589-93. doi: 10.2460/javma.239.12.1589.
7
Simplicimonas-like DNA in vaginal swabs of cows and heifers cross-reacting in the real-time PCR for T. foetus.奶牛和小母牛阴道拭子中与胎儿三毛滴虫实时PCR发生交叉反应的类单形滴虫属DNA。
Vet Parasitol. 2017 Apr 15;237:30-36. doi: 10.1016/j.vetpar.2017.02.024. Epub 2017 Feb 24.
8
Detection of by RT-rtPCR in pooled bovine preputial washings.应用 RT-rtPCR 技术对牛包皮冲洗液进行 的检测。
J Vet Diagn Invest. 2023 Mar;35(2):178-181. doi: 10.1177/10406387221149407. Epub 2023 Jan 10.
9
Use of pooled protozoal cultures of preputial scraping samples obtained from bulls for the detection of Tritrichomonas foetus by means of a real-time polymerase chain reaction assay.使用从公牛包皮刮取样本中获得的原虫混合培养物，通过实时聚合酶链反应测定法检测胎儿三毛滴虫。
J Am Vet Med Assoc. 2014 Feb 1;244(3):352-6. doi: 10.2460/javma.244.3.352.
10
Addressing the constraints of Tritrichomonas foetus sample collection in remote areas: lyophilized modified Diamond's media as a substitute for liquid medium.解决偏远地区阴道毛滴虫样本采集的限制：冻干改良 Diamond 培养基可替代液体培养基。
Parasitology. 2019 Aug;146(9):1184-1187. doi: 10.1017/S0031182019000258. Epub 2019 Mar 12.
本文引用的文献
1
Time and temperature stability of in phosphate-buffered saline as evaluated by a reverse transcription real-time PCR assay and field analysis.通过逆转录实时聚合酶链反应分析和现场分析评估在磷酸盐缓冲盐水中的时间和温度稳定性。
Front Vet Sci. 2023 Mar 30;10:1101502. doi: 10.3389/fvets.2023.1101502. eCollection 2023.
2
Control and eradication of bovine trichomonosis in Wyoming, USA by testing and culling positive bulls.通过检测和淘汰阳性公牛，实现美国怀俄明州牛滴虫病的控制和根除。
Vet Res. 2021 Oct 7;52(1):129. doi: 10.1186/s13567-021-00996-w.
3
Improvements in Tritrichomonas foetus molecular testing.胎儿三毛滴虫分子检测的改进
J Vet Diagn Invest. 2018 Jul;30(4):603-608. doi: 10.1177/1040638718767943. Epub 2018 Apr 10.
4
Effects of bacterial contamination of media on the diagnosis of Tritrichomonas foetus by culture and real-time PCR.培养基细菌污染对通过培养和实时荧光定量PCR诊断胎儿三毛滴虫的影响。
Vet Parasitol. 2015 Mar 15;208(3-4):143-9. doi: 10.1016/j.vetpar.2015.01.006. Epub 2015 Jan 19.
5
Evaluation of effects of high incubation temperatures on results of protozoal culture and real-time PCR testing for Tritrichomonas foetus inoculated in a commercially available self-contained culture media system.评估高温孵育对接种于市售独立培养基系统中的胎儿三毛滴虫原虫培养结果及实时聚合酶链反应检测结果的影响。
J Am Vet Med Assoc. 2011 Dec 15;239(12):1589-93. doi: 10.2460/javma.239.12.1589.
6
The influence of temperature and simulated transport conditions of diagnostic samples on real-time polymerase chain reaction for the detection of Tritrichomonas foetus DNA.诊断样本的温度和模拟运输条件对检测胎儿三毛滴虫DNA的实时聚合酶链反应的影响。
J Vet Diagn Invest. 2011 Sep;23(5):982-5. doi: 10.1177/1040638711417143.
7
Tritrichomonas foetus.胎儿三毛滴虫
Vet Clin North Am Food Anim Pract. 2006 Nov;22(3):595-611. doi: 10.1016/j.cvfa.2006.07.001.
8
Improved detection of Tritrichomonas foetus in bovine diagnostic specimens using a novel probe-based real time PCR assay.使用基于新型探针的实时PCR检测方法改进牛诊断样本中胎儿三毛滴虫的检测。
Vet Parasitol. 2006 Nov 5;141(3-4):204-15. doi: 10.1016/j.vetpar.2006.06.012. Epub 2006 Jul 24.
9
Effects of temperature on the survival of Tritrichomonas foetus in transport, Diamond's and InPouch TF media.温度对胎儿三毛滴虫在运输、戴蒙德培养基和InPouch TF培养基中存活的影响。
Vet Rec. 1999 Feb 27;144(9):227-32. doi: 10.1136/vr.144.9.227.
10
Pathogenesis, diagnosis, and management of trichomoniasis in cattle.
Vet Clin North Am Food Anim Pract. 1997 Jul;13(2):345-61. doi: 10.1016/s0749-0720(15)30346-7.
Zotero 插件