Zhang Longfei, Liu Xiaoxiao, Xu Mingze, Cheng Xinyi, Li Ning, Xu Haiyan, Feng Yining, Guan Tianzhu, Xiao Lixia
College of Food Science and Technology, Yangzhou University, Yangzhou 225000, China.
Jiangsu Provincial Key Laboratory for Probotics and Dairy Deep Processing, Yangzhou 225000, China.
Foods. 2025 Mar 25;14(7):1145. doi: 10.3390/foods14071145.
L. (), a traditional food and medicinal plant, is used to treat internal inflammation. This study investigated the mechanisms by which improves ulcerative colitis (UC) via combined UHPLC-OE-MS/MS, network pharmacology, molecular docking, and animal experiments. A total of 72 compounds were detected in the extraction, with 15 key components (ranking by degree value) selected for further analysis. GO enrichment analysis suggested that PS may alleviate UC-related renal dysfunction by modulating immune responses, inflammation, and cell signaling pathways. Based on protein-protein interaction results, five core targets of in UC (ranking by degree value) were identified, and molecular docking revealed strong binding free affinity (<-7 kcal/mol) of active components (Vulgarin and 4-(Diphenylphosphino)benzoic acid) with TNF, AKT1, CASP3, BCL2, and MMP9. In animal experiments, -treated mice showed significant reductions in IL-6, TNF-α, LPS, and D-Lactate levels ( < 0.05); improved colon histopathological damage; and significantly increased the mRNA expression of tight junction proteins (ZO-1, Claudin, OCC) in colon tissue ( < 0.05). Furthermore, -treated mice exhibited a significant increase in beneficial gut bacteria ( and ) ( < 0.05), effectively restoring the gut imbalance caused by DSS. In conclusion, can treat UC through the modulation of the intestinal microecology.
L.（一种传统的食用和药用植物）用于治疗体内炎症。本研究通过UHPLC - OE - MS/MS联用、网络药理学、分子对接和动物实验，探究了其改善溃疡性结肠炎（UC）的机制。在该提取物中总共检测到72种化合物，选择了15种关键成分（按度值排名）进行进一步分析。基因本体（GO）富集分析表明，该植物可能通过调节免疫反应、炎症和细胞信号通路来减轻UC相关的肾功能障碍。基于蛋白质 - 蛋白质相互作用结果，确定了该植物在UC中的五个核心靶点（按度值排名），分子对接显示活性成分（缬草素和4 - （二苯基膦基）苯甲酸）与肿瘤坏死因子（TNF）、蛋白激酶B1（AKT1）、半胱天冬酶3（CASP3）、B细胞淋巴瘤2（BCL2）和基质金属蛋白酶9（MMP9）具有很强的结合自由亲和力（<-7千卡/摩尔）。在动物实验中，该植物处理的小鼠白细胞介素 - 6（IL - 6）、肿瘤坏死因子 - α（TNF - α）、脂多糖（LPS）和D - 乳酸水平显著降低（P<0.05）；结肠组织病理损伤得到改善；结肠组织中紧密连接蛋白（闭合蛋白1（ZO - 1）、闭合蛋白（Claudin）、闭锁小带蛋白（OCC））的mRNA表达显著增加（P<0.05）。此外，该植物处理的小鼠有益肠道细菌（双歧杆菌和乳酸菌）显著增加（P<0.05），有效恢复了由葡聚糖硫酸钠（DSS）引起的肠道失衡。总之，该植物可通过调节肠道微生态来治疗UC。
