Cercamondi Colin I, Bendik Igor, Eckhardt Erik, Mak Tim, Seifert Nicole, Kuratli Karin, Richard Nathalie, Tamasi Balint, Mussler Bernd, Wintergerst Eva
DSM-Firmenich AG, Wurmisweg 576, 4303 Kaiseraugst, Switzerland.
Foods. 2025 Mar 27;14(7):1173. doi: 10.3390/foods14071173.
Postbiotics may help strengthen intestinal barrier function. This study assessed the effects of a postbiotic derived from and subsp. on epithelial barrier and cytokine production. Human-derived colon tubules were cultured on chips for 15 days. On day 8, the epithelial barrier was disrupted with 0.7 μM afatinib. Postbiotic doses of 5, 10, or 20 mg/mL were added on days 6, 8, 11, and 13. Trans-epithelial electrical resistance (TEER) was measured on days 6, 8, 11, 13, and 15, along with phase contrast imaging. Cytokine levels were measured on day 13. All three postbiotic concentrations resulted in better TEER recovery on day 15 vs. the control ( < 0.001). On day 13, 10 and 20 mg/mL increased TEER ( < 0.001), but only 20 mg/mL did on day 11 ( < 0.05). Phase imaging confirmed the dose-dependent effect. The 20 mg/mL dose more effectively reduced CCL2, CX3CL1, CXCL1, CXCL5, IL-8, IL-11, and IL-4 than the other doses ( < 0.01), and 10 mg/mL more effectively reduced CCL2, CXCL1, CXCL10, IL-10, IL-11, and IL-23 than 5 mg/mL ( < 0.01). In a colonic organoid model, the -derived postbiotic prevented drug-induced epithelial damage, enhanced recovery, and modulated cytokine secretion towards a more anti-inflammatory profile in a dose-dependent manner.
后生元可能有助于增强肠道屏障功能。本研究评估了一种源自[具体名称1]和[具体名称2]亚种的后生元对上皮屏障和细胞因子产生的影响。将人源结肠小管在芯片上培养15天。在第8天，用0.7 μM阿法替尼破坏上皮屏障。在第6、8、11和13天添加5、10或20 mg/mL的后生元剂量。在第6、8、11、13和15天测量跨上皮电阻（TEER），并进行相差成像。在第13天测量细胞因子水平。与对照组相比，所有三种后生元浓度在第15天均导致更好的TEER恢复（P<0.001）。在第13天，10和20 mg/mL增加了TEER（P<0.001），但在第11天只有20 mg/mL增加了TEER（P<0.05）。相差成像证实了剂量依赖性效应。与其他剂量相比，20 mg/mL剂量更有效地降低了CCL2、CX3CL1、CXCL1、CXCL5、IL-8、IL-11和IL-4（P<0.01），10 mg/mL比5 mg/mL更有效地降低了CCL2、CXCL1、CXCL10、IL-10、IL-11和IL-23（P<0.01）。在结肠类器官模型中，源自[具体名称1]的后生元可预防药物诱导的上皮损伤，增强恢复，并以剂量依赖性方式调节细胞因子分泌，使其向更具抗炎性的方向发展。
