Kono Takashi, Matsuda Tatsuma, Fujimoto Masanori, Taki Yuki, Sakuma Ikki, Hashimoto Naoko, Nakamura Yasuhiro, Horiguchi Kentaro, Higuchi Yoshinori, Onodera Atsushi, Miki Takashi, Tanaka Tomoaki
Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba 260-8677, Japan; Research Institute of Disaster Medicine, Chiba University, Chiba 260-8677, Japan.
Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba 260-8677, Japan; Department of Neurological Surgery Chiba University Graduate School of Medicine, Chiba 260-8677, Japan.
STAR Protoc. 2025 Apr 14;6(2):103760. doi: 10.1016/j.xpro.2025.103760.
Here, we present a comprehensive protocol for analyzing craniopharyngioma subtypes and their tumor microenvironments at single-cell resolution. We describe tumor tissue dissociation, single-cell isolation, RNA sequencing library preparation, and bioinformatics analysis procedures. We also detail immunohistochemistry procedures for validating findings. This approach enables the detailed characterization of tumor cellular composition, immune cell networks, and molecular signatures that distinguish adamantinomatous from papillary craniopharyngiomas. For complete details on the use and execution of this protocol, refer to Matsuda et al..
在此，我们展示了一种用于在单细胞分辨率下分析颅咽管瘤亚型及其肿瘤微环境的综合方案。我们描述了肿瘤组织解离、单细胞分离、RNA测序文库制备和生物信息学分析程序。我们还详细介绍了用于验证结果的免疫组织化学程序。这种方法能够详细表征肿瘤细胞组成、免疫细胞网络以及区分成釉细胞瘤型和乳头型颅咽管瘤的分子特征。有关此方案使用和执行的完整详细信息，请参考松田等人的研究。
