Sajwan Reena K, Solanki Pratima R
Nano-Bio Laboratory, Special Centre for Nanoscience, Jawaharlal Nehru University, New Delhi, 110067, India.
Nano-Bio Laboratory, Special Centre for Nanoscience, Jawaharlal Nehru University, New Delhi, 110067, India.
Talanta. 2025 Oct 1;293:128057. doi: 10.1016/j.talanta.2025.128057. Epub 2025 Apr 2.
The antibiotic residue in food accelerates bacteria's resistance process that is responsible for serious health issues. A smartphone-integrated ratiometric colorimetric aptasensor has been developed for ampicillin (AMP) detection in milk and egg samples. The sensing phenomenon utilizes the optical and color-changing properties of gold nanoparticles (AuNPs) and specific aptamer with higher affinity towards AMP for its selective detection. The aptasensor shows linear response with AMP concentration 0.06-9.9 μM with a lower detection limit (LOD) of 45.0 nM. The smartphone-integrated color analyzer application was used to identify the solution's red, green, and blue (RGB) color intensity and calculate the G/R ratio of color intensities in the presence of AMP. Aptasensor show linear response with AMP concentration (0-6.6 μM), with a LOD of 53.0 nM. The sensor's results were further validated with liquid chromatography and mass spectroscopy techniques (LC-MS), showing that the developed sensor is suitable for food monitoring and safety applications.
食品中的抗生素残留会加速细菌的抗药过程，这会引发严重的健康问题。一种集成智能手机的比率比色适体传感器已被开发用于检测牛奶和鸡蛋样本中的氨苄青霉素（AMP）。传感现象利用了金纳米颗粒（AuNPs）的光学和颜色变化特性以及对AMP具有更高亲和力的特异性适体来进行选择性检测。该适体传感器对AMP浓度在0.06 - 9.9 μM范围内呈线性响应，检测限（LOD）为45.0 nM。集成智能手机的颜色分析仪应用程序用于识别溶液的红、绿、蓝（RGB）颜色强度，并计算存在AMP时颜色强度的G/R比率。适体传感器对AMP浓度在（0 - 6.6 μM）范围内呈线性响应，LOD为53.0 nM。传感器的结果通过液相色谱和质谱技术（LC-MS）进一步验证，表明所开发的传感器适用于食品监测和安全应用。
