Chen Yiming, Kong Dehui, Wang Zongkuan, Liu Jiaqian, Wang Linghan, Dai Keli, Ji Jialun, Chen Wei, Tang Xiong, Wen Mingxing, Zhang Xu, Zhang Huajian, Jiao Chengzhi, Sun Li, Wang Haiyan, Fei Xingru, Guo Hong, Sun Bingjian, Tao Xiaorong, Wang Wei, Yang Jian, Wang Xiue, Xiao Jin
National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization/Zhongshan Biological Breeding Laboratory/CIC-MCP, Nanjing, Jiangsu, China.
Institute of Plant Virology, Ningbo University, Ningbo, Zhejiang, China.
Nat Commun. 2025 Apr 16;16(1):3630. doi: 10.1038/s41467-025-58816-0.
Ym1 is the most widely utilized gene for wheat yellow mosaic virus (WYMV) disease control in worldwide wheat breeding. Here, we successfully isolated the responsible gene for Ym1. It encodes a typical CC-NBS-LRR type R protein, which is specifically expressed in root and induced upon WYMV infection. Ym1-mediated WYMV resistance is likely achieved by blocking viral transmission from the root cortex into steles, thereby preventing systemic movement to aerial tissues. Ym1 CC domain is essential for triggering cell death. Ym1 specifically interacts with WYMV coat protein, and this interaction leads to nucleocytoplasmic redistribution, a process for transitioning Ym1 from an auto-inhibited to an activated state. The activation subsequently elicits hypersensitive responses and establishes WYMV resistance. Ym1 is likely introgressed from the sub-genome X or X of polyploid Aegilops species. The findings highlight an exogenous-introgressed and root-specifically expressed R gene that confers WYMV resistance by recognizing the viral component.
Ym1是全球小麦育种中用于控制小麦黄花叶病毒（WYMV）病害应用最为广泛的基因。在此，我们成功分离出了Ym1的相关基因。它编码一种典型的CC-NBS-LRR型R蛋白，该蛋白在根中特异性表达，并在WYMV侵染时被诱导。Ym1介导的对WYMV的抗性可能是通过阻断病毒从根皮层向中柱的传播来实现的，从而防止病毒向地上组织的系统移动。Ym1的CC结构域对于触发细胞死亡至关重要。Ym1与WYMV外壳蛋白特异性相互作用，这种相互作用导致核质重新分布，这是一个使Ym1从自抑制状态转变为激活状态的过程。随后这种激活引发超敏反应并建立对WYMV的抗性。Ym1可能是从多倍体节节麦属物种的亚基因组X或X渗入而来。这些发现凸显了一个外源渗入且在根中特异性表达的R基因，该基因通过识别病毒成分赋予对WYMV的抗性。
