Agricultural Research Department, Hokkaido Research Organization, Chuo Agricultural Experiment Station, Higashi-6, Kita-15, Naganuma, Hokkaido, 069-1395, Japan,
Theor Appl Genet. 2015 Aug;128(8):1569-78. doi: 10.1007/s00122-015-2532-y. Epub 2015 May 10.
Wheat yellow mosaic virus resistance of Madsen is governed by two complementary QTLs, Qym1 and Qym2 , located on chromosome arms 2DL and 3BS. Wheat yellow mosaic, caused by Wheat yellow mosaic virus (WYMV), is one of the most serious wheat diseases in East Asia. In this study, recombinant inbred lines (RILs, F9) from a cross between cultivars Madsen (resistant) and Hokushin (susceptible) grown in a WYMV-infected nursery field were tested for the presence of WYMV in leaves by enzyme-linked immunosorbent assay (ELISA) and genotyped by using genome-wide molecular markers. Two major QTLs were detected: Qym1 located between Xgwm539 and Xgwm349 on chromosome 2DL and Qym2 located between Xbarc147 and Xwmc623 on chromosome 3BS. The resistance alleles for both QTLs originated from Madsen. The third QTL Qym3 located near Xwmc457 on chromosome 4D, where the resistant allele for this QTL originated from Hokushin. Although the Qym3 was rather minor, it was essential to complement Qym1 and Qym2 for complete avoidance of WYMV infection. Near-isogenic lines carrying the resistance QTLs were developed by repeated backcrosses using Madsen as the donor parent and Hokushin as the recurrent parent. The lines that were resistant to WYMV (as tested by ELISA) were homozygous for the Madsen alleles at both Qym1 and Qym2. Qym1 dominance was partial, whereas that of Qym2 was nearly complete. Qym1 was closely linked to Xwmc41; Qym2 was closely linked to Xwmc754. These markers will be useful in marker-assisted selection in wheat breeding for WYMV resistance; this study will facilitate cloning the WYMV resistance genes.
小麦黄花叶病毒抗性由 Madsen 中的两个互补 QTL 控制，分别位于染色体臂 2DL 和 3BS 上的 Qym1 和 Qym2。由小麦黄花叶病毒（WYMV）引起的小麦黄花叶病是东亚地区小麦最严重的病害之一。在这项研究中，在 WYMV 感染的苗圃地种植 Madsen（抗性）和 Hokushin（感病）品种的杂交后代重组自交系（RIL，F9），通过酶联免疫吸附测定（ELISA）检测叶片中是否存在 WYMV，并利用全基因组分子标记进行基因型分析。检测到两个主要的 QTL：位于染色体 2DL 上的 Xgwm539 和 Xgwm349 之间的 Qym1 和位于染色体 3BS 上的 Xbarc147 和 Xwmc623 之间的 Qym2。这两个 QTL 的抗性等位基因均来自 Madsen。第三个 QTL Qym3 位于染色体 4D 上的 Xwmc457 附近，该 QTL 的抗性等位基因来自 Hokushin。虽然 Qym3 较小，但对于完全避免 WYMV 感染，它是与 Qym1 和 Qym2 互补所必需的。通过使用 Madsen 作为供体亲本和 Hokushin 作为轮回亲本进行多次回交，开发了携带抗性 QTL 的近等基因系。通过 ELISA 测试对 WYMV 具有抗性的系在 Qym1 和 Qym2 处均为 Madsen 等位基因纯合。Qym1 的显性是部分的，而 Qym2 的则几乎是完全的。Qym1 与 Xwmc41 紧密连锁；Qym2 与 Xwmc754 紧密连锁。这些标记将有助于小麦抗 WYMV 育种中的标记辅助选择；本研究将有助于克隆 WYMV 抗性基因。
