Wang Tao, Zhu Bolin, Lu Jianyu, Guo Xinya, Li Ruikang, Yuan Yue, Chen Junjie, Dai Xi, Liu Shuai, Du Jiaxin, Xu Xun, Liu Huan, Wei Xiaoyu, Huang Runzhi, Ji Shizhao
College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.
BGI Research, Hangzhou, 310030, China.
Sci Data. 2025 Apr 16;12(1):639. doi: 10.1038/s41597-025-04928-7.
Thermal injuries represent one of the most severe forms of trauma to the human body, with a high annual incidence of burn victims globally. Skin regeneration and wound healing following thermal injury constitute a complex process involving various cell types and cytokine interactions. By Single-cell ATAC sequencing (scATAC-seq), we elucidated the molecular mechanisms underlying the dermal regeneration and healing processes following thermal injury in a rat model. Tissue samples were harvested for sequencing at predetermined intervals (0 h, 12 h, 24 h, 3 d, 7 d, 11 d, 15 d, and 19 d post-injury), yielding 28,179 high-quality single cells. Our comprehensive analysis revealed 28 distinct cell populations throughout the regenerative process, encompassing various subsets of keratinocytes, fibroblasts, and immune cells, exhibiting temporal heterogeneity across samples. Furthermore, we investigated the chromatin accessibility landscape of individual cell types and identified enriched transcription factor binding motifs, corroborating the robustness and validity of our data. Our dataset provides a valuable resource for further elucidation of burnt skin regeneration and healing processes.
热损伤是人体最严重的创伤形式之一，全球每年有大量烧伤患者。热损伤后的皮肤再生和伤口愈合是一个复杂的过程，涉及多种细胞类型和细胞因子相互作用。通过单细胞ATAC测序（scATAC-seq），我们阐明了大鼠模型热损伤后真皮再生和愈合过程的分子机制。在预定时间点（损伤后0小时、12小时、24小时、3天、7天、11天、15天和19天）采集组织样本进行测序，共获得28179个高质量单细胞。我们的综合分析揭示了整个再生过程中的28个不同细胞群，包括角质形成细胞、成纤维细胞和免疫细胞的各个亚群，不同样本间呈现出时间异质性。此外，我们研究了单个细胞类型的染色质可及性图谱，并确定了富集的转录因子结合基序，证实了我们数据的稳健性和有效性。我们的数据集为进一步阐明烧伤皮肤再生和愈合过程提供了宝贵资源。
