HOXD1调控绵羊神经嵴细胞分化和多角发育。

HOXD1 regulates neural crest cells differentiation and polycerate development in sheep.

作者信息

Zhang Cheng, Zhang Shuhong, Xu Huifeng, Wang Guan, Zhang Huan, Di Tenggang, Tian Liming, Chang Menghan, Gao Fengyi, Li Ming, Yang Guangli

机构信息

College of Biology and Food, Shangqiu Normal University, Shangqiu, 476000, China.

College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450002, China.

出版信息

Sci Rep. 2025 Apr 16;15(1):13140. doi: 10.1038/s41598-025-97865-9.

DOI:10.1038/s41598-025-97865-9

PMID:40240791

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12003793/

Abstract

The polycerate trait in sheep is a complex phenotype regulated by polygenes. However, the mechanism behind multi-horned traits development and growth remains unclear. In this study, neural crest cells (NCCs) were isolated from mouse embryos, and the HOXD1 (Homeobox D1) gene was overexpressed in these cells to identify its function. Transcriptome analysis was performed to explore the key signaling pathway involved in forming the multi-horned traits in sheep. The results showed that the HOXD1 induced epithelial-to-mesenchymal transition (EMT) in mouse neural crest primary cells, affecting their migration but without significantly influencing proliferation. Furthermore, signaling pathway analysis suggested that HOXD1 may inhibit NCC proliferation by modulating Wnt rather than TGF-β signaling. Transcriptome analysis revealed that the HOXD1 gene affected the extracellular matrix of CXC family regulatory cells and promoted NCC differentiation. These findings provide a theoretical basis for further investigation into the regulation of multi-horned traits growth and development in sheep.

摘要

绵羊的多角性状是一种受多基因调控的复杂表型。然而，多角性状发育和生长背后的机制仍不清楚。在本研究中，从小鼠胚胎中分离出神经嵴细胞（NCCs），并在这些细胞中过表达HOXD1（同源框D1）基因以确定其功能。进行转录组分析以探索参与绵羊多角性状形成的关键信号通路。结果表明，HOXD1在小鼠神经嵴原代细胞中诱导上皮-间质转化（EMT），影响其迁移，但对增殖没有显著影响。此外，信号通路分析表明，HOXD1可能通过调节Wnt而非TGF-β信号通路来抑制NCC增殖。转录组分析显示，HOXD1基因影响CXC家族调节细胞的细胞外基质并促进NCC分化。这些发现为进一步研究绵羊多角性状生长发育的调控提供了理论依据。