Tountas C P, Bergman R A
J Hand Surg Am. 1977 Jan;2(1):31-7. doi: 10.1016/s0363-5023(77)80007-5.
Biopsies of muscle from ten patients and of muscle and nerve from three monkeys were obtained during periods of continuous ischemia and studied by light and electron microscopy and by histochemical means. Between the third and fourth hours of ischemia, swelling of the mitochondria was noted in monkey muscle. One week later the appearance was normal. In the monkey nerves degenerated myelin was seen one week later but appeared to be normal at 2 weeks. If after 3 hours the tourniquet was deflated for 30 minutes and then reapplied for 2 additional hours, no changes were found. Succinic dehyrogenase analysis was used to identify swelling of the mitochondria. No mitochondrial changes were seen in human muscle made ischemic by a tourniquet for 2 hours.
在持续缺血期间，获取了10例患者的肌肉活检标本以及3只猴子的肌肉和神经活检标本，并通过光学显微镜、电子显微镜和组织化学方法进行研究。在缺血的第3至4小时之间，观察到猴子肌肉中的线粒体肿胀。一周后外观正常。在猴子神经中，一周后可见髓鞘变性，但在2周时似乎正常。如果在3小时后松开止血带30分钟，然后再重新绑扎2小时，则未发现变化。采用琥珀酸脱氢酶分析来识别线粒体肿胀。用止血带使人体肌肉缺血2小时，未观察到线粒体变化。
