Vukmirović Antonia, Škvorc Željko, Bogdan Saša, Krstonošić Daniel, Bogdan Ida Katičić, Karažija Tomislav, Bačurin Marko, Brener Magdalena, Sever Krunoslav
Faculty of Forestry and Wood Technology, University of Zagreb, Svetošimunska Cesta 23, HR-10000 Zagreb, Croatia.
Faculty of Agriculture, University of Zagreb, Svetošimunska Cesta 25, HR-10000 Zagreb, Croatia.
Int J Mol Sci. 2025 Mar 26;26(7):3053. doi: 10.3390/ijms26073053.
During drought, a major abiotic stressor for European forests, excessive reactive oxygen species (ROS) are produced, causing oxidative damage that affects structural and metabolic tree functions. This research examines the effects of drought, phosphorus (P) fertilization, and provenance on photosynthetic pigments, malondialdehyde (MDA) concentrations, and antioxidant enzyme activities in common beech ( L.) and sessile oak ( (Matt.) Liebl.) saplings from two provenances. In a common garden experiment, four treatments were applied: regular watering with (+PW) and without P fertilization (-PW), and drought with (+PD) and without (-PD) P fertilization. Results showed that drought increased both MDA concentrations and antioxidant enzyme activity, particularly superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX), which are responsible for ROS scavenging. Additionally, chlorophyll + concentrations were lower in drought-exposed plants. Phosphorus fertilization minimally affected MDA levels but enhanced antioxidant responses, particularly APX and CAT activities in oak during drought. Provenance differences were notable, with oak and beech from the drier provenance showing better adaptation, reflected in lower MDA levels and higher enzyme activities. This study underscores the importance of antioxidant defenses in coping with drought stress, with phosphorus fertilization and provenance shaping the species' adaptive capacity.
在干旱期间，干旱是欧洲森林面临的主要非生物胁迫因素，会产生过量的活性氧（ROS），导致氧化损伤，影响树木的结构和代谢功能。本研究考察了干旱、磷肥施用和种源对来自两个种源的欧洲山毛榉（Fagus sylvatica L.）和无梗花栎（Quercus petraea (Matt.) Liebl.）幼苗光合色素、丙二醛（MDA）浓度和抗氧化酶活性的影响。在一个共同园试验中，设置了四种处理：定期浇水且施磷（+PW）、定期浇水不施磷（-PW）、干旱且施磷（+PD）、干旱不施磷（-PD）。结果表明，干旱会增加MDA浓度和抗氧化酶活性，尤其是超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）和抗坏血酸过氧化物酶（APX），这些酶负责清除ROS。此外，遭受干旱的植株中叶绿素a + b浓度较低。磷肥施用对MDA水平影响最小，但增强了抗氧化反应，尤其是干旱期间栎树中的APX和CAT活性。种源差异显著，来自较干燥种源的栎树和山毛榉表现出更好的适应性，表现为较低的MDA水平和较高的酶活性。本研究强调了抗氧化防御在应对干旱胁迫中的重要性，磷肥施用和种源塑造了物种的适应能力。
