Like phytohormones, peptide hormones participate in many cellular processes, participate in intercellular communications, and are involved in signal transmission. The system of intercellular communications based on peptide-receptor interactions plays a critical role in the development and functioning of plants. One of the most important molecules are reactive oxygen species (ROS). ROS participate in signaling processes and intercellular communications, including the development of the root system. ROS are recognized as active regulators of cell division and differentiation, which depend on the oxidation-reduction balance. The stem cell niche and the size of the root meristem are maintained by the intercellular interactions and signaling networks of peptide hormone and ROS. Therefore, peptides and ROS can interact with each other both directly and indirectly and function as regulators of cellular processes. Peptides and ROS regulate cell division and stem cell differentiation through a negative feedback mechanism. In this review, we focused on the molecular mechanisms regulating the development of the main root, lateral roots, and nodules, in which peptides and ROS participate.