Genome-Wide Identification and Expression Analysis Unveil the Involvement of the Succinic Semialdehyde Dehydrogenase () Gene Family in Banana Low Temperature Stress.

Banana ( spp.) is susceptible to low-temperature stress and other environmental stresses, which can hinder the growth and development. Succinic semialdehyde dehydrogenase () is critical for GABA biosynthesis and plays a crucial role in plants. However, the genes of bananas have not been studied. This study found 19 , 18 , and 18 from the banana genome. According to the phylogenetic tree, these genes can be categorized into five branches. This study cloned the from banana. The subcellular localization assays of in tobacco leaves confirmed that the presence of was not only localized mitochondrion but also localized chloroplast. The -elements of the gene family are related to the potential regulation of the banana gene family; their involvement in diverse stress responses. Transcriptomic data was utilized to examine the effect of genes under cold stress in bananas. The results of RT-qPCR were consistent with transcriptome data. These results showed that most are passively responsive to low-temperature treatment. In addition, transient overexpression of in leaves resulted in the content of GABA increasing, indicating that may be involved in the accumulation of GABA of banana. Collectively, these results improve knowledge of the gene family in banana and establish a basis for comprehending its biological roles in response to low temperatures.